Hidrolisat protein kelas kultur sel C-Cell
Hidrolisat protein nabati dan ragi ultrafiltrasi berendotoksin rendah buatan Organotechnie, Prancis, untuk kultur bebas serum dan rendah serum sel CHO, HEK293, Vero, sel punca mesenkimal, dan sel serangga. STBIO MEDIA adalah distributor di Taiwan yang menyediakan sampel, spesifikasi, dan COA per batch.
Fetal bovine serum is expensive, variable between batches and carries animal-origin risk, so vaccine, monoclonal antibody, cell therapy and cultivated meat processes all look for ways to use less of it. Cell-culture-grade protein hydrolysates are the most common replacement supplement: plant or yeast protein is cut into peptides and amino acids, ultrafiltered to remove large molecules and controlled for endotoxin, so it can supply part of the nutrition and growth factors serum used to provide.
C-Cell is the range Organotechnie designed for cell culture, split by raw material into soy, pea, guar, broad bean, rice, wheat and yeast grades, each with validation data on CHO, HEK293 or Vero. STBIO MEDIA has distributed the range since 2023, holds stock in Taiwan and provides free samples.
Produk dalam rangkaian
| C-Cell | Bahan baku | Kegunaan | Divalidasi pada | |
|---|---|---|---|---|
| S104 Soy C-Cell S104 | Soy | Ultrafiltered, low-endotoxin soy hydrolysate for mammalian cell proliferation. | HEK293, CHO | Halaman produk |
| S146B Soy C-Cell S146B | Soy | For CHO cell proliferation and protein expression; supports partial replacement of fetal bovine serum. | CHO | Halaman produk |
| S186 Soy C-Cell S186 | Soy | For CHO cell proliferation and protein expression; supports partial replacement of fetal bovine serum. | CHO | Halaman produk |
| S204 Soy C-Cell S204 | Soy | Soy hydrolysate; one of the three best performers in the HEK293 recombinant protein study. | HEK293 | Tanya harga |
| P112 Pea C-Cell P112 | Pea | Pea hydrolysate for CHO proliferation and protein expression; free of soy and gluten allergens. | CHO, HEK293 | Halaman produk |
| G115 Guar C-Cell G115 | Guar | Guar hydrolysate; the manufacturer reports it promotes enzyme protein expression in mammalian cells. | HEK293, CHO | Halaman produk |
| F120 Broad Bean C-Cell F120 | Broad bean | Broad bean hydrolysate for CHO cell proliferation. | CHO | Halaman produk |
| R116 Rice C-Cell R116 | Rice | Rice protein hydrolysate, low allergen, for CHO cell proliferation. | CHO | Halaman produk |
| R123 Rice C-Cell R123 | Rice | Rice protein hydrolysate, low allergen, for CHO cell proliferation. | CHO | Halaman produk |
| W106 Wheat C-Cell W106 | Wheat | Ultrafiltered wheat peptone for cell culture and animal-vaccine microbial fermentation; the best wheat source in the Vero serum-reduction study. | Vero, HEK293 | Tanya harga |
| Y109 Yeast C-Cell Y109 | Yeast | Ultrafiltered yeast hydrolysate for mammalian and insect cell proliferation and protein expression. | CHO, HEK293, Sf9 | Halaman produk |
| Y121 Yeast C-Cell Y121 | Yeast | Ultrafiltered yeast hydrolysate for mammalian and insect cell culture. | CHO, Sf9 | Halaman produk |
Organotechnie® and C-Cell are trademarks of Organotechnie S.A.S., France. ST BIO, INC. is their distributor in Taiwan; the products are manufactured by Organotechnie.
Galur sel dan aplikasi
- CHOCHO cells: monoclonal antibodies and recombinant proteinsAdded to serum-free or low-serum basal media to supply peptides and growth factors, supporting cell density and product accumulation. Suspension cultures usually take a higher dose than adherent ones. Most C-Cell grades are validated primarily on CHO.S104S146BS186P112G115F120R116R123Y109Y121
- HEK293HEK293 transient expressionAn Organotechnie R&D report expressed IL-4 in HEK293-6E and compared plant-derived C-Cell grades with Tryptone N1; P112, G115 and S204 gave markedly higher expression than the control.S104S204P112G115W106Y109
- VeroVero cells and serum reduction for vaccine productionA study by Inserm and Organotechnie achieved the same Vero proliferation with 5% serum plus 0.5 g/L peptone as with 10% serum, halving serum use.W106
- MSCMesenchymal stromal cells and extracellular vesicle productionAn Inserm study coated culture surfaces with plant peptone and used EV-depleted fetal bovine serum to raise extracellular vesicle yield from umbilical cord MSCs while retaining function.
- Sf9Insect cells and baculovirus expressionYeast-derived Y109 and Y121 are suited to proliferation and protein expression in Sf9 and other insect cells.Y109Y121
Pertanyaan umum
01What is C-Cell?
C-Cell is the cell-culture-grade protein hydrolysate range from Organotechnie S.A.S., France: plant or yeast proteins enzymatically hydrolysed and ultrafiltered, with low endotoxin, used to supply peptides and growth factors in serum-free or low-serum media. STBIO MEDIA is the distributor in Taiwan.
02How does C-Cell differ from an ordinary peptone?
Three things: ultrafiltration (removing large molecules and potential viral or endotoxin load), an endotoxin specification, and performance testing on cell lines. Ordinary fermentation peptones lack these and often inhibit growth or introduce endotoxin when added to cell culture media.
03What dose should I start with?
It varies widely with the cell type and culture mode. In published studies, adherent Vero cells used 0.5 g per litre (0.05%) while suspension HEK293 used 0.5% (5 g per litre), a tenfold difference. Trial three levels between 0.25 and 5 g per litre, starting low for adherent cells and high for suspension cells, then read cell density and product.
04Can it fully replace serum?
It depends on the cell line. Published data show serum can be cut from 10% to 5% in Vero and partially replaced in CHO; fully serum-free culture usually needs an optimised basal medium and adaptation. We supply samples so you can verify in your own system.
05What documentation is available?
Each batch comes with a COA (including endotoxin), a TDS and an SDS; the manufacturer can provide animal-origin, non-GMO and process statements. Contact us for documents required for regulatory filings.
06Is it animal-origin-free?
Plant- and yeast-derived C-Cell grades contain no animal-derived raw material; the origin of enzymes and processing aids is stated in the manufacturer's declaration, available with the sample.
07Minimum order and lead time?
Samples are free; order sizes follow the pack formats. Common grades are stocked in Taiwan and the rest ship from France. Tell us the cell line, application and volume via the quotation form.
Laporan penelitian
- HEK293 recombinant protein expression: plant peptones vs Tryptone N1Organotechnie R&D report
- Vero cell proliferation and serum reductionPoster, Inserm U1197 and Organotechnie
- Optimising EV production from umbilical cord MSCsPoster, Inserm U1197
- Trends in plant and microbial hydrolysates for cell cultureVirginia Tech study
The following are summaries, written in our own words, of research reports published independently by their authors and institutions. Results belong to the original reports and are not product claims by STBIO MEDIA.
Estimasi pengurangan serum
Masukkan volume dan harga Anda sendiri untuk melihat selisih tahunan bila serum dikurangi dan C-Cell ditambahkan. Semua angka dari Anda; situs ini tidak mengasumsikan harga.
- Serum yang dihemat per tahun
- 50 L
- C-Cell yang dibutuhkan
- 0,5 kg
- Biaya serum yang dihemat
- —
- Biaya C-Cell
- —
- Selisih bersih per tahun
- —
Masukkan harga serum dan C-Cell untuk melihat jumlahnya. Hanya estimasi; tidak termasuk biaya media, tenaga kerja, dan validasi. Kelayakan pengurangan serum harus dikonfirmasi pada galur sel Anda. Jumlah dalam mata uang yang Anda masukkan.