示意圖，僅供參考，商品以實物為準Burkholderia Cepacia Selective Agar Base
洋蔥伯克霍爾德氏菌選擇性瓊脂基礎培養基(BCSA)
別名與縮寫：BCSA Base · Burkholderia Cepacia Agar Base · BCSA · Burkholderia cepacia Selective Agar
For the selective isolation of Burkholderia cepacia complex from non-sterile pharmaceutical products and other materials, following the addition of a selective supplement.
- 類別
- 粉末培養基
- 每公升溶解量
- 50.58 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-13
產品說明
原理
Burkholderia Cepacia Selective Agar was developed by Henry and co-workers as an improvement on earlier selective media, giving faster growth and better selectivity for the Burkholderia cepacia complex.¹ Pancreatic digest of casein and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Most strains of the Burkholderia cepacia complex oxidise these sugars slowly and release alkaline end-products from the peptones, so that colonies and the surrounding medium turn pink to red after prolonged incubation. Selectivity is provided by crystal violet, which inhibits Gram-positive organisms, vancomycin, which inhibits enterococci and other Gram-positive organisms not suppressed by crystal violet, and polymyxin B with gentamicin, which act together against other aerobic Gram-negative bacilli including Pseudomonas. The medium is specified in the pharmacopoeia for the examination of non-sterile products for the Burkholderia cepacia complex.²
配製與使用
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
培養性能測試
| 測試菌株 | ATCC | 生長情形 |
|---|---|---|
| Burkholderia cepacia | 25416 | good-luxuriant |
| Pseudomonas aeruginosa | 9027 | inhibited |
| Escherichia coli | 8739 | inhibited |
| Staphylococcus aureus | 6538 | inhibited |
產品特色
- 依標準配方製造，每公升溶解量 50.58 g
- 出廠前以標準菌株驗證培養效能（Burkholderia cepacia、Pseudomonas aeruginosa、Escherichia coli、Staphylococcus aureus）
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 50.58 g；配製與滅菌條件依規格書。
02適用哪些菌種？
革蘭氏陰性菌、病原菌、好氧菌、Burkholderia cepacia、Pseudomonas aeruginosa、Escherichia coli、Staphylococcus aureus。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Henry D. A., Campbell M. E., LiPuma J. J. and Speert D. P., 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J. Clin. Microbiol., 35:614-619.
- 2. United States Pharmacopeia, current edition. Chapter <60> Microbiological Examination of Nonsterile Products — Tests for Burkholderia cepacia Complex, USP-NF, Rockville, MD.