示意圖，僅供參考，商品以實物為準ATCC Medium 271
ATCC 271 號培養基(大腸桿菌培養基)
別名與縮寫：Escherichia Medium · ATCC 271 Medium · E. coli K-12 Medium
For the cultivation of Escherichia coli K-12 strains, as recommended by the American Type Culture Collection.
- 類別
- 粉末培養基
- 每公升溶解量
- 20.3 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-06
產品說明
原理
The medium is the formulation recommended by the American Type Culture Collection for the cultivation of Escherichia coli K-12 and its derivatives.¹ Tryptone and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, dextrose provides a readily fermentable carbon and energy source, and sodium chloride maintains the osmotic equilibrium. Calcium chloride supplies calcium ions, and thiamine supports the growth of thiamine-requiring K-12 derivatives commonly used in laboratory work. The heat-sensitive components are supplied separately and added after sterilisation.
配製與使用
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
培養性能測試
| 測試菌株 | ATCC | 生長情形 |
|---|---|---|
| Escherichia coli K-12 | 10798 | good-luxuriant |
產品特色
- 依標準配方製造，每公升溶解量 20.3 g
- 出廠前以標準菌株驗證培養效能（Escherichia coli K-12）
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 20.3 g；配製與滅菌條件依規格書。
02適用哪些菌種？
革蘭氏陰性菌、好氧菌、大腸桿菌、Escherichia coli K-12。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. American Type Culture Collection, Catalogue of Bacteria and Bacteriophages, ATCC, Manassas, VA.