示意圖,僅供參考,商品以實物為準Pseudomonas Agar Base
假單胞菌瓊脂基礎培養基
別名與縮寫:Pseudomonas CFC Agar Base · Pseudomonas CN Agar Base · Pseudomonas Selective Agar Base · CFC Agar Base · 假單胞菌洋菜基礎 · CN / CFC 洋菜基礎
For the isolation and enumeration of Pseudomonas aeruginosa in water and of Pseudomonas species in meat, milk, and dairy products, following the addition of glycerol and a selective supplement.
- 類別
- 粉末培養基
- 每公升溶解量
- 48.4 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新:2026-11-06
產品說明
原理
The base is derived from King's Medium A and contains gelatin peptone and casein hydrolysate as sources of nitrogen, amino acids, and growth factors, with glycerol as the carbon and energy source. Potassium sulphate and magnesium chloride enhance the production of pigments by Pseudomonas aeruginosa. With the CFC supplement, cetrimide, fusidic acid, and cefalotin suppress the competing flora of meat and dairy products, allowing the selective enumeration of Pseudomonas species, which are important spoilage organisms of chilled foods.¹ With the CN supplement, the higher concentration of cetrimide together with nalidixic acid makes the medium selective for Pseudomonas aeruginosa, which is enumerated in water by membrane filtration.² Colonies producing the blue-green pigment pyocyanin are regarded as confirmed Pseudomonas aeruginosa, while fluorescent colonies without pyocyanin require further confirmation. The medium is specified in ISO 16266 for water, ISO 13720 for meat, and ISO/TS 11059 for milk and milk products.³
配製與使用
Suspend 48.4 g of powder in 1 L of purified water containing 10 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the CFC selective supplement for Pseudomonas species, or the CN selective supplement for Pseudomonas aeruginosa. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
培養性能測試
| 測試菌株 | ATCC | 生長情形 |
|---|---|---|
| Pseudomonas fluorescens | 13525 | good-luxuriant |
| Pseudomonas aeruginosa | 27853 | good-luxuriant |
| Pseudomonas aeruginosa | 27853 | good-luxuriant |
| Escherichia coli | 25922 | inhibited |
產品特色
- 依標準配方製造,每公升溶解量 48.4 g
- 出廠前以標準菌株驗證培養效能(Pseudomonas fluorescens、Pseudomonas aeruginosa、Pseudomonas aeruginosa、Escherichia coli)
- 500 g / 5 kg / 25 kg 三種以上包裝,研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表,支援樣品申請
- 台灣製造,供貨穩定
常見問題
01每公升要加多少克粉末?
每公升去離子水加 48.4 g;配製與滅菌條件依規格書。
02適用哪些菌種?
革蘭氏陰性菌、嗜冷菌、病原菌、綠膿桿菌、Pseudomonas fluorescens、Pseudomonas aeruginosa、Escherichia coli。詳見培養性能表與規格書。
03這個產品要怎麼配製?
Suspend 48.4 g of powder in 1 L of purified water containing 10 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the CFC selective supplement for *Pseudomonas* species, or the CN selective supplement for *Pseudomonas aeruginosa*. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價?可以提供 COA 嗎?
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格,請於詢價時說明應用場景與用量,一個工作天內回覆,並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎?交期多久?
本產品在新北市汐止的自有工廠生產、台灣出貨,不需進口與清關。交期依庫存與包裝規格而定,詢價時告知品項與用量,我們會一併回覆現貨狀況與交期;急件請在詢價單註明。
06可以開立統一發票嗎?學校與研究機構可以請購嗎?
可以。我們開立台灣統一發票(含營業稅),學校、研究機構與公家單位可依請購流程先索取報價單再下單;小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎?
有。規格書(TDS)與 SDS 以英文版為標準文件,登入後可在產品頁下載;中文版 SDS 依需求提供,請在詢價單或聯絡表單註明品項與用途,我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Mead G. C. and Adams B. W., 1977. A selective medium for the rapid isolation of pseudomonads associated with poultry meat spoilage. Br. Poult. Sci., 18:661-670.
- 2. ISO 16266, Water quality — Detection and enumeration of Pseudomonas aeruginosa — Method by membrane filtration, International Organization for Standardization, Geneva.
- 3. ISO 13720, Meat and meat products — Enumeration of presumptive Pseudomonas spp., International Organization for Standardization, Geneva.
