STBIO MEDIA上鼎生技有限公司上鼎醱酵培養基有限公司
Brilliant Green Agar (Modified)
示意圖,僅供參考,商品以實物為準
REF. CM1206

Brilliant Green Agar (Modified)

改良亮綠瓊脂培養基(Edel-Kampelmacher 培養基)

別名與縮寫:BGA (Mod.) · Edel-Kampelmacher Medium · Modified Brilliant Green Agar · BPLS Agar · Brilliant-green Phenol-red Lactose Sucrose Agar · 改良亮綠洋菜 · Edel-Kampelmacher 培養基

For the selective isolation of Salmonella species, other than Salmonella Typhi, from water, meat, milk, and other materials.

類別
粉末培養基
每公升溶解量
51.7 g
包裝規格
500 g · 5 kg · 25 kg

資料更新:2026-11-06

包裝規格
1
業務將於 1 個工作天內回覆報價索取免費樣品

產品說明

Brilliant Green Agar (Modified) 是瓊脂(固態)培養基,屬於食品與臨床檢驗用的選擇性培養基,以選擇性成分抑制雜菌、以鑑別成分辨識目標菌落。以煌綠抑制大多數雜菌,用於沙門氏菌的分離。上鼎以食品級原料製造,生產環境通過 ISO 22000 認證,每批附 COA。

原理

Brilliant Green Agar (Modified) is based on the formulation of Edel and Kampelmacher, who added a phosphate buffer and reduced the brilliant green concentration of Kauffmann's medium to improve the recovery of Salmonella in a standardised isolation procedure.¹ Peptone, meat extract, and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and the phosphate buffer maintains the pH of the medium. Brilliant green inhibits Gram-positive bacteria and most Gram-negative bacilli other than Salmonella. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Salmonella does not ferment either sugar and forms red colonies surrounded by a bright red zone, whereas lactose- or sucrose-fermenting organisms produce acid and form yellow-green colonies with a yellow zone. The medium is specified in ISO 6340 for the detection of Salmonella in water and in DIN 10160 and DIN 10182.²

選擇性培養基的檢驗流程

病原菌檢驗通常分三段:先用增菌培養基(如緩衝蛋白腖水)讓受損菌體復原並增加數量,再用選擇性增菌培養基抑制雜菌,最後用選擇性鑑別培養基分離出目標菌落。選擇性成分多為膽鹽、染劑或抗生素,用來抑制非目標菌;鑑別成分則讓目標菌長出特定顏色或型態的菌落。請依您採用的檢驗方法選擇對應的培養基組合。

配製與使用

Suspend 51.7 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.

培養性能測試

測試菌株ATCC生長情形
Salmonella enterica ser. Typhimurium14028good-luxuriant
Salmonella enterica ser. Enteritidis13076good-luxuriant
Escherichia coli25922inhibited-partial
Staphylococcus aureus25923inhibited

產品特色

  • 依標準配方製造,每公升溶解量 51.7 g
  • 出廠前以標準菌株驗證培養效能(Salmonella enterica ser. Typhimurium、Salmonella enterica ser. Enteritidis、Escherichia coli、Staphylococcus aureus)
  • 500 g / 5 kg / 25 kg 三種以上包裝,研究室到工業規模都適用
  • 可提供 COA、規格書與技術資料表,支援樣品申請
  • 台灣製造,供貨穩定

常見問題

01選擇性培養基可以高壓滅菌嗎?

不一定。部分含有染劑或選擇性抑制劑的培養基過度加熱會失去選擇性,只需加熱至完全溶解、不可高壓滅菌。請依本產品的配製說明操作,不確定時歡迎聯絡我們確認。

02每公升要加多少克粉末?

每公升去離子水加 51.7 g;配製與滅菌條件依規格書。

03適用哪些菌種?

腸桿菌科、革蘭氏陰性菌、病原菌、沙門氏菌、Salmonella enterica ser. Typhimurium、Salmonella enterica ser. Enteritidis、Escherichia coli、Staphylococcus aureus。詳見培養性能表與規格書。

04這個產品要怎麼配製?

Suspend 51.7 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.

05大量採購如何詢價?可以提供 COA 嗎?

可以。本產品提供 500 g、5 kg、25 kg 等包裝規格,請於詢價時說明應用場景與用量,一個工作天內回覆,並可提供 COA、規格書與技術資料表。

06台灣有現貨嗎?交期多久?

本產品在新北市汐止的自有工廠生產、台灣出貨,不需進口與清關。交期依庫存與包裝規格而定,詢價時告知品項與用量,我們會一併回覆現貨狀況與交期;急件請在詢價單註明。

07可以開立統一發票嗎?學校與研究機構可以請購嗎?

可以。我們開立台灣統一發票(含營業稅),學校、研究機構與公家單位可依請購流程先索取報價單再下單;小包裝與樣品申請都接受。

08有中文版的 SDS 與規格書嗎?

有。規格書(TDS)與 SDS 以英文版為標準文件,登入後可在產品頁下載;中文版 SDS 依需求提供,請在詢價單或聯絡表單註明品項與用途,我們會隨報價寄出。每批出貨附 COA。

參考文獻

  1. 1. Edel W. and Kampelmacher E. H., 1969. Salmonella isolation in nine European laboratories using a standardized technique. Bull. World Health Organ., 41:297-306.
  2. 2. ISO 6340, Water quality — Detection of Salmonella spp., International Organization for Standardization, Geneva.
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