示意圖，僅供參考，商品以實物為準Muller-Kauffmann Tetrathionate-Novobiocin Broth Base
穆勒-考夫曼四硫磺酸鹽新生黴素液體基礎培養基(MKTTn)
別名與縮寫：MKTTn · MKTTn Broth Base · Muller-Kauffmann Tetrathionate Broth Base · MKTT Broth Base · Tetrathionate Broth Base (Muller-Kauffmann) · MKTTn 培養液 · 四硫磺酸鹽新生黴素增菌液 · ISO 6579 沙門氏菌增菌液
For the selective enrichment of Salmonella species from food, animal feed, and environmental samples, following the addition of iodine-iodide solution and novobiocin.
- 類別
- 粉末培養基
- 每公升溶解量
- 89.49 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-07
產品說明
Muller-Kauffmann Tetrathionate-Novobiocin Broth Base（穆勒－考夫曼四硫磺酸鹽新生黴素培養液基礎，MKTTn）是 ISO 6579-1 指定的沙門氏菌選擇性增菌液之一：使用前加入碘液生成四硫磺酸鹽，膽鹽與煌綠抑制雜菌，另加新生黴素抑制變形桿菌，與 RVS 培養液並行增菌後分別劃線 XLD 與第二種分離平板。配方與台灣食藥署公告方法、FDA BAM 的四硫代硫酸鹽培養液（TT）不同，做公告方法請依方法原文配製。基礎配方，碘液與新生黴素依方法添加。
原理
Tetrathionate broth was introduced by Muller and modified by Kauffmann, who added brilliant green and ox bile to increase its selectivity for Salmonella.¹ʼ² Meat extract and enzymatic digest of casein supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. On addition of the iodine-iodide solution, sodium thiosulphate is oxidised to tetrathionate. Organisms possessing tetrathionate reductase, including Salmonella, are able to utilise tetrathionate, whereas most competing enteric bacteria are inhibited. Calcium carbonate neutralises the acid produced during the reduction of tetrathionate. Ox bile and brilliant green inhibit Gram-positive bacteria and many coliforms, and novobiocin suppresses Proteus species, which also reduce tetrathionate. Enrichment cultures are subcultured onto selective plating media for isolation.³
配製與使用
Suspend 89.39 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. DO NOT AUTOCLAVE. Cool below 45°C and aseptically add 20 mL of iodine-iodide solution and novobiocin to a final concentration of 40 mg/L. Mix well and dispense into sterile tubes or flasks while swirling to keep the calcium carbonate in suspension. Use the complete medium on the day of preparation. Test the finished medium for performance using stable, standard control cultures.
產品特色
- 依標準配方製造，每公升溶解量 89.49 g
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 89.49 g；配製與滅菌條件依規格書。
02適用哪些菌種？
腸桿菌科、革蘭氏陰性菌、病原菌、沙門氏菌。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 89.39 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. DO NOT AUTOCLAVE. Cool below 45°C and aseptically add 20 mL of iodine-iodide solution and novobiocin to a final concentration of 40 mg/L. Mix well and dispense into sterile tubes or flasks while swirling to keep the calcium carbonate in suspension. Use the complete medium on the day of preparation. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Muller L., 1923. Un nouveau milieu d'enrichissement pour la recherche du bacille typhique et des paratyphiques. C. R. Soc. Biol. (Paris), 89:434-437.
- 2. Kauffmann F., 1935. Weitere Erfahrungen mit dem kombinierten Anreicherungsverfahren für Salmonellabacillen. Z. Hyg. Infektionskr., 117:26-32.
- 3. ISO 6579-1, Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 1: Detection of Salmonella spp., International Organization for Standardization, Geneva.