示意圖，僅供參考，商品以實物為準WL Nutrient Agar
瓦勒斯坦營養瓊脂培養基
別名與縮寫：WLN · WL 營養洋菜 · 釀造微生物培養基
For the cultivation and differentiation of yeasts and other microorganisms in brewing, winemaking, and other fermentation processes.
- 類別
- 粉末培養基
- 每公升溶解量
- 80.25 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-06
產品說明
WL Nutrient Agar 是瓊脂（固態）培養基，屬於通用與計數類的標準配方。用於非挑剔性細菌的培養、計數與菌種保存，是實驗室最基本的固態培養基。上鼎以食品級原料製造，生產環境通過 ISO 22000 認證，每批附 COA。
原理
WL Nutrient Agar was developed by Green and Gray at the Wallerstein Laboratories for the microbiological control of brewing and other fermentation processes.¹ Yeast extract and pancreatic digest of casein supply nitrogen, amino acids, vitamins, and growth factors, and dextrose serves as the carbon and energy source. The mineral salts provide the ions required by yeasts and other fermentation organisms, and potassium dihydrogen phosphate buffers the medium. Bromocresol green acts as a pH indicator and is taken up by colonies to differing degrees, so that organisms develop characteristic colours, ranging from white and cream to various shades of green, together with distinctive colony morphologies. These differences allow culture yeasts to be distinguished from wild yeasts and other contaminants. The medium supports both yeasts and bacteria; for the enumeration of bacteria in the presence of yeasts, WL Differential Agar, which contains cycloheximide, is used.²
通用與計數培養基怎麼選
平板計數培養基用於食品、飲用水與環境檢體的總生菌數計數；營養培養基與胰化大豆培養基是非挑剔性細菌的通用選擇，後者營養較豐富，也用於無菌試驗與環境監控；腦心浸出液培養基營養最豐富，適合鏈球菌等營養需求較高的菌種。需要計數就用瓊脂版，需要增殖就用液體版。
配製與使用
Suspend 80.252 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
培養性能測試
| 測試菌株 | ATCC | 生長情形 |
|---|---|---|
| Saccharomyces cerevisiae | 9763 | good-luxuriant |
| Saccharomyces pastorianus | 9080 | good-luxuriant |
| Lactiplantibacillus plantarum | 8014 | good |
產品特色
- 依標準配方製造，每公升溶解量 80.25 g
- 出廠前以標準菌株驗證培養效能（Saccharomyces cerevisiae、Saccharomyces pastorianus、Lactiplantibacillus plantarum）
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01總生菌數計數要用哪一種培養基？
食品與飲用水的總生菌數計數通常使用平板計數培養基（PCA），以傾注平板法於 30°C 培養。實際培養溫度與時間請依您採用的檢驗方法規定。
02每公升要加多少克粉末？
每公升去離子水加 80.25 g；配製與滅菌條件依規格書。
03適用哪些菌種？
酵母菌、乳酸菌、啤酒酵母、乳酸桿菌、Saccharomyces cerevisiae、Saccharomyces pastorianus、Lactiplantibacillus plantarum。詳見培養性能表與規格書。
04這個產品要怎麼配製？
Suspend 80.252 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
05大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
06台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
07可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
08有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
學術研究引用
本產品已被國立臺灣大學研究團隊引用於同儕審查期刊，作為微生物培養實驗的培養基來源：
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
培養基來源標注：Tryptone Soy Broth、MRS medium、LB broth、beef extract、peptone、yeast extract、agar（STBIO, New Taipei, Taiwan）
查看論文全文參考文獻
- 1. Green S. R. and Gray P. P., 1950. A differential procedure applicable to bacteriological investigation in brewing. Wallerstein Lab. Commun., 13:357-366.
- 2. American Society of Brewing Chemists, Methods of Analysis, Microbiological Control, ASBC, St. Paul, MN.