示意圖，僅供參考，商品以實物為準Legionella CYE Agar Base
活性碳酵母抽出物瓊脂基礎培養基
別名與縮寫：CYE / BCYE · CYE Agar Base · Charcoal Yeast Extract Agar Base · Legionella Agar Base · BCYE Agar Base · 退伍軍人菌培養基 · BCYE 洋菜基礎
For the isolation and enumeration of Legionella species from water and environmental samples, following the addition of BCYE growth supplement and, where required, a selective supplement.
- 類別
- 粉末培養基
- 每公升溶解量
- 25 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-06
產品說明
Legionella CYE Agar Base（活性碳酵母抽出物培養基基礎，CYE／BCYE）是退伍軍人菌（Legionella pneumophila）分離培養的基礎：酵母抽出物提供生長所需，活性碳吸附培養基中對退伍軍人菌有毒的過氧化物與自由基，使用前加入 ACES 緩衝、L-半胱胺酸與焦磷酸鐵補充物即成 BCYE，需要選擇性時再加抗生素。ISO 11731 冷卻水塔、熱水系統與醫院用水檢驗指定使用。基礎配方，補充物依方法添加。
原理
Charcoal Yeast Extract Agar was developed by Feeley and co-workers as a primary isolation medium for Legionella pneumophila, and was later improved by the addition of ACES buffer and α-ketoglutarate.¹ʼ² Yeast extract supplies nitrogen, vitamins, and growth factors. Activated charcoal detoxifies the medium by adsorbing inhibitory compounds and decomposing hydrogen peroxide and other toxic oxygen derivatives formed during preparation and incubation. The growth supplement provides the specific requirements of Legionella: L-cysteine, an essential amino acid for the genus, and ferric pyrophosphate as a soluble iron source. ACES buffer maintains the narrow pH range on which growth depends, and α-ketoglutarate stimulates growth. For samples with a high background flora, selective agents such as glycine, vancomycin, polymyxin B, and cycloheximide are added. Legionella colonies are grey-white and show a characteristic ground-glass appearance under a stereomicroscope.³
配製與使用
Suspend 25 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted BCYE growth supplement and, for contaminated samples, the selective supplement. Mix well and pour into sterile Petri plates, swirling continuously to keep the charcoal in suspension. Test the finished medium for performance using stable, standard control cultures.
產品特色
- 依標準配方製造，每公升溶解量 25 g
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 25 g；配製與滅菌條件依規格書。
02適用哪些菌種？
病原菌、革蘭氏陰性菌、好氧菌、環境監控。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 25 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted BCYE growth supplement and, for contaminated samples, the selective supplement. Mix well and pour into sterile Petri plates, swirling continuously to keep the charcoal in suspension. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C. and Baine W. B., 1979. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J. Clin. Microbiol., 10:437-441.
- 2. Edelstein P. H., 1981. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J. Clin. Microbiol., 14:298-303.
- 3. ISO 11731, Water quality — Enumeration of Legionella, International Organization for Standardization, Geneva.