示意圖，僅供參考，商品以實物為準Giolitti-Cantoni Broth Base
GC 增菌液體基礎培養基
別名與縮寫：GC Broth · Giolitti-Cantoni Broth · Giolitti and Cantoni Broth · Giolitti-Cantoni Staphylococcus Enrichment Broth · GC 增菌液 · 金黃色葡萄球菌增菌液
For the selective enrichment of Staphylococcus aureus from food, following the addition of potassium tellurite.
- 類別
- 粉末培養基
- 每公升溶解量
- 54.2 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-06
產品說明
Giolitti-Cantoni Broth Base（GC 增菌液基礎）是金黃色葡萄球菌最確數法與低菌量檢體的選擇性增菌液：氯化鋰與使用前加入的亞碲酸鉀抑制雜菌，甘露醇與丙酮酸鈉幫助受損的葡萄球菌復原，培養後變黑或產生黑色沉澱即為推定陽性，再劃線 Baird-Parker 平板確認。ISO 6888-3 指定使用，適合乳粉、嬰兒食品與加工後菌數偏低的檢體。基礎配方，亞碲酸鉀依方法添加。
原理
Giolitti-Cantoni Broth was described by Giolitti and Cantoni for the enrichment of staphylococci from foods.¹ Tryptone, meat extract, and yeast extract supply nitrogen, amino acids, vitamins, and growth factors. Mannitol, glycine, and sodium pyruvate stimulate the growth of Staphylococcus aureus; sodium pyruvate also degrades hydrogen peroxide and assists the recovery of sub-lethally injured cells. Lithium chloride and potassium tellurite inhibit Gram-negative bacteria and most Gram-positive organisms other than Staphylococcus aureus. Sealing the tubes with paraffin creates anaerobic conditions that suppress micrococci, while the facultatively anaerobic Staphylococcus aureus continues to grow. Staphylococcus aureus reduces tellurite to metallic tellurium, producing blackening of the medium, which is taken as a presumptive positive result. Blackened tubes are subcultured onto Baird-Parker Agar for confirmation.²
配製與使用
Suspend 54.2 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Cool and aseptically add sterile potassium tellurite solution to the concentration specified in the applicable method. After inoculation, seal each tube with a layer of sterile molten paraffin or agar. Test the finished medium for performance using stable, standard control cultures.
產品特色
- 依標準配方製造，每公升溶解量 54.2 g
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 54.2 g；配製與滅菌條件依規格書。
02適用哪些菌種？
病原菌、革蘭氏陽性菌、金黃色葡萄球菌。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 54.2 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Cool and aseptically add sterile potassium tellurite solution to the concentration specified in the applicable method. After inoculation, seal each tube with a layer of sterile molten paraffin or agar. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Giolitti G. and Cantoni C., 1966. A medium for the isolation of staphylococci from foodstuffs. J. Appl. Bacteriol., 29:395-398.
- 2. ISO 6888-3, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci — Part 3: Detection and MPN technique for low numbers, International Organization for Standardization, Geneva.