示意圖，僅供參考，商品以實物為準m-FC Agar
m-FC 糞便大腸菌群瓊脂培養基
別名與縮寫：m-FC · mFC Agar · M-FC Agar · Fecal Coliform Agar · m FC Agar Base · Fecal Coliforms Agar · m-FC 洋菜 · 糞便大腸桿菌群濾膜培養基
For the detection and enumeration of fecal coliforms in water by the membrane filtration technique.
- 類別
- 粉末培養基
- 每公升溶解量
- 52.1 g
- 包裝規格
- 500 g · 5 kg · 25 kg
資料更新：2026-11-07
產品說明
m-FC Agar（m-FC 糞便大腸桿菌群培養基）是濾膜法檢測水中糞便大腸桿菌群的培養基：以 44.5°C 的高溫培養搭配玫瑰紅酸與膽鹽選擇，糞便大腸桿菌群發酵乳糖使苯胺藍指示劑轉藍，長成藍色菌落。APHA 水質標準方法 9222D 指定使用，是河川、養殖用水、遊憩水域與廢水處理放流水監測的標準項目。含染劑，加熱溶解後不可高壓滅菌。
原理
m-FC Agar was developed by Geldreich and co-workers for the enumeration of fecal coliforms by membrane filtration without a preliminary enrichment step.¹ Tryptose, proteose peptone, and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose is the fermentable carbohydrate. Bile salts inhibit Gram-positive organisms, and rosolic acid suppresses the background growth of non-coliform bacteria. Membranes are incubated at 44.5°C, a temperature at which only thermotolerant (fecal) coliforms grow well. Fecal coliforms ferment lactose, and the acid produced reacts with aniline blue so that colonies appear in various shades of blue; non-fecal coliforms and other organisms form grey to cream-coloured colonies. Blue colonies are counted and the result is expressed as fecal coliforms per 100 mL of sample.²
配製與使用
Suspend 52.1 g of powder in 1 L of purified water containing 10 mL of a 1% solution of rosolic acid in 0.2 N sodium hydroxide. Heat with continuous stirring and bring to the boil for 1 minute. DO NOT AUTOCLAVE. Cool to 45-50°C and dispense into 50-60 mm Petri plates. Test the finished medium for performance using stable, standard control cultures.
培養性能測試
| 測試菌株 | ATCC | 生長情形 |
|---|---|---|
| Escherichia coli | 25922 | good-luxuriant |
| Klebsiella aerogenes | 13048 | inhibited-partial |
| Enterococcus faecalis | 29212 | inhibited |
產品特色
- 依標準配方製造，每公升溶解量 52.1 g
- 出廠前以標準菌株驗證培養效能（Escherichia coli、Klebsiella aerogenes、Enterococcus faecalis）
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
常見問題
01每公升要加多少克粉末？
每公升去離子水加 52.1 g；配製與滅菌條件依規格書。
02適用哪些菌種？
大腸桿菌群、革蘭氏陰性菌、大腸桿菌、Escherichia coli、Klebsiella aerogenes、Enterococcus faecalis。詳見培養性能表與規格書。
03這個產品要怎麼配製？
Suspend 52.1 g of powder in 1 L of purified water containing 10 mL of a 1% solution of rosolic acid in 0.2 N sodium hydroxide. Heat with continuous stirring and bring to the boil for 1 minute. DO NOT AUTOCLAVE. Cool to 45-50°C and dispense into 50-60 mm Petri plates. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Geldreich E. E., Clark H. F., Huff C. B. and Best L. C., 1965. Fecal-coliform-organism medium for the membrane filter technique. J. Am. Water Works Assoc., 57:208-214.
- 2. American Public Health Association, Standard Methods for the Examination of Water and Wastewater, current edition, APHA Press, Washington, D.C.