Leptospira Medium Base EMJH
鉤端螺旋體 EMJH 基礎培養基
別名與縮寫：EMJH Medium Base · Ellinghausen-McCullough-Johnson-Harris Medium Base · EMJH Base
For the cultivation and maintenance of Leptospira species, following the addition of Leptospira Enrichment EMJH.
- 類別
- 粉末培養基
- 每公升溶解量
- 2.55 g
- 包裝規格
- 500 g · 5 kg · 25 kg
- 認證
- ISO 22000
資料更新：2026-11-06
- 保存條件
- Store at 10–30 °C in sealed, dry, clean conditions.
- 保存期限
- 36 months from manufacture date when stored as directed.
產品說明
原理
The medium is based on the oleic acid–albumin formulation of Ellinghausen and McCullough, as modified by Johnson and Harris.¹ʼ² The base supplies phosphate buffer, sodium chloride for osmotic balance, ammonium chloride as the nitrogen source, and thiamine, a vitamin required by leptospires. Leptospira species cannot utilise carbohydrates and depend on long-chain fatty acids as their source of carbon and energy. These are provided by polysorbate 80 in the enrichment, while bovine serum albumin binds the free fatty acids and releases them gradually, protecting the organisms from their toxic effect.¹ The enrichment also supplies vitamin B12 and the trace metals required for growth. Growth is slow and does not produce turbidity in the early stages; cultures are examined periodically by dark-field microscopy for motile spirochaetes.²
配製與使用
Suspend 2.3 g of powder in 900 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to room temperature and aseptically add 100 mL of Leptospira Enrichment EMJH. Mix well and dispense into sterile tubes. For a semi-solid medium, add 1.5 g of agar per litre before heating. Test the finished medium for performance using stable, standard control cultures.
產品特色
- 食品級原料，生產環境通過 ISO 22000 食品安全管理系統認證
- 依標準配方製造，每公升溶解量 2.55 g
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
食品級與實驗室級的差別
市面上多數培養基供應商使用實驗室級（laboratory grade）原料，在重金屬殘留、微生物污染管控與原料來源追溯上的標準較低，用於純研究足夠，但不適合對原料品質有更高要求的場景。上鼎的產品以食品級（food-grade）原料製造，生產全程在通過 ISO 22000 食品安全管理系統認證的設施內進行。
- 食品微生物檢測實驗室，需符合 ISO 17025 或相關法規要求
- 生技廠商生產供出口的發酵產品，需完整原料溯源文件
- 對供應鏈有 ESG 或合規審查要求的機構
- 同時採購清真與非清真產品、需統一原料等級標準的企業
常見問題
01每公升要加多少克粉末？
每公升去離子水加 2.55 g；配製與滅菌條件依規格書。
02適用哪些菌種？
病原菌、革蘭氏陰性菌、好氧菌。詳見培養性能表與規格書。
03可以用於食品級用途嗎？
可以。本產品以食品級原料製造，生產環境通過 ISO 22000 食品安全管理系統認證，適用於直接食用的發酵產品的菌種培養。
04這個產品要怎麼配製？
Suspend 2.3 g of powder in 900 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to room temperature and aseptically add 100 mL of Leptospira Enrichment EMJH. Mix well and dispense into sterile tubes. For a semi-solid medium, add 1.5 g of agar per litre before heating. Test the finished medium for performance using stable, standard control cultures.
05開封後要怎麼保存？有效期限多久？
Store at 10–30 °C in sealed, dry, clean conditions. 36 months from manufacture date when stored as directed.
06大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
07台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
08可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
09有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Ellinghausen H. C. and McCullough W. G., 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res., 26:45-51.
- 2. Johnson R. C. and Harris V. G., 1967. Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures. J. Bacteriol., 94:27-31.