画像はイメージです。実際の製品とは異なる場合があります。Burkholderia Cepacia Selective Agar Base
別名・略称：BCSA Base · Burkholderia Cepacia Agar Base · BCSA · Burkholderia cepacia Selective Agar
For the selective isolation of Burkholderia cepacia complex from non-sterile pharmaceutical products and other materials, following the addition of a selective supplement.
- 分類
- 乾燥培地
- 1 L あたり溶解量
- 50.58 g
- 包装
- 500 g · 5 kg · 25 kg
データ更新日：2026-11-13
製品概要
原理
Burkholderia Cepacia Selective Agar was developed by Henry and co-workers as an improvement on earlier selective media, giving faster growth and better selectivity for the Burkholderia cepacia complex.¹ Pancreatic digest of casein and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Most strains of the Burkholderia cepacia complex oxidise these sugars slowly and release alkaline end-products from the peptones, so that colonies and the surrounding medium turn pink to red after prolonged incubation. Selectivity is provided by crystal violet, which inhibits Gram-positive organisms, vancomycin, which inhibits enterococci and other Gram-positive organisms not suppressed by crystal violet, and polymyxin B with gentamicin, which act together against other aerobic Gram-negative bacilli including Pseudomonas. The medium is specified in the pharmacopoeia for the examination of non-sterile products for the Burkholderia cepacia complex.²
調製方法
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
培養性能試験
| 試験菌株 | ATCC | 発育 |
|---|---|---|
| Burkholderia cepacia | 25416 | 良好〜旺盛 |
| Pseudomonas aeruginosa | 9027 | 発育抑制 |
| Escherichia coli | 8739 | 発育抑制 |
| Staphylococcus aureus | 6538 | 発育抑制 |
製品の特長
- 標準組成に基づいて製造、1 Lあたり50.58 g
- 出荷前に標準対照菌株による性能を確認（Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus）
- 500 g / 5 kg / 25 kgでご用意、試験室規模から工業規模まで対応
- 試験成績書（COA）、規格書、技術データシート（TDS）をご提供可能、サンプルはご要望に応じて対応
- 台湾製、安定供給
よくあるご質問
011 L あたり何 g の粉末を加えますか？
脱イオン水 1 L あたり 50.58 g を加えます。調製・滅菌条件は規格書をご参照ください。
02どの菌種に適していますか？
Gram-negative, Pathogens, Aerobes, Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus。詳細は発育性能試験表と規格書をご参照ください。
03この製品はどのように調製しますか？
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04大口のお見積りはどのように依頼すればよいですか？また、COAは提供してもらえますか？
はい。本製品は500 g, 5 kg, 25 kgでご用意しています。お見積りのご依頼時に用途と数量をお知らせください。1営業日以内にご返信し、試験成績書（COA）、規格書、技術データシート（TDS）をご提供できます。
参考文献
- 1. Henry D. A., Campbell M. E., LiPuma J. J. and Speert D. P., 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J. Clin. Microbiol., 35:614-619.
- 2. United States Pharmacopeia, current edition. Chapter <60> Microbiological Examination of Nonsterile Products — Tests for Burkholderia cepacia Complex, USP-NF, Rockville, MD.