Gambar hanya ilustrasi. Produk aktual dapat berbeda.Burkholderia Cepacia Selective Agar Base
Dikenal juga sebagai：BCSA Base · Burkholderia Cepacia Agar Base · BCSA · Burkholderia cepacia Selective Agar
For the selective isolation of Burkholderia cepacia complex from non-sterile pharmaceutical products and other materials, following the addition of a selective supplement.
- Kategori
- Media Kultur Kering
- Larutkan per liter
- 50.58 g
- Ukuran kemasan
- 500 g · 5 kg · 25 kg
Data diperbarui：2026-11-13
Ringkasan
Prinsip
Burkholderia Cepacia Selective Agar was developed by Henry and co-workers as an improvement on earlier selective media, giving faster growth and better selectivity for the Burkholderia cepacia complex.¹ Pancreatic digest of casein and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Most strains of the Burkholderia cepacia complex oxidise these sugars slowly and release alkaline end-products from the peptones, so that colonies and the surrounding medium turn pink to red after prolonged incubation. Selectivity is provided by crystal violet, which inhibits Gram-positive organisms, vancomycin, which inhibits enterococci and other Gram-positive organisms not suppressed by crystal violet, and polymyxin B with gentamicin, which act together against other aerobic Gram-negative bacilli including Pseudomonas. The medium is specified in the pharmacopoeia for the examination of non-sterile products for the Burkholderia cepacia complex.²
Petunjuk penyiapan
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Respons kultur
| Organisme uji | ATCC | Pertumbuhan |
|---|---|---|
| Burkholderia cepacia | 25416 | baik-subur |
| Pseudomonas aeruginosa | 9027 | terhambat |
| Escherichia coli | 8739 | terhambat |
| Staphylococcus aureus | 6538 | terhambat |
Keunggulan produk
- Diproduksi sesuai formulasi standar, 50.58 g per liter
- Kinerja diverifikasi terhadap galur kontrol standar sebelum dirilis (Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus)
- Tersedia dalam 500 g / 5 kg / 25 kg, dari skala laboratorium hingga industri
- Tersedia COA, lembar spesifikasi, dan lembar data teknis; sampel dapat diminta
- Diproduksi di Taiwan dengan pasokan yang andal
Pertanyaan yang sering diajukan
01Berapa gram bubuk per liter?
Gunakan 50.58 g per liter air deionisasi; kondisi penyiapan dan sterilisasi tercantum pada lembar spesifikasi.
02Cocok untuk mikroorganisme apa?
Gram-negative, Pathogens, Aerobes, Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus. Lihat tabel uji kinerja pertumbuhan dan lembar spesifikasi.
03Bagaimana cara menyiapkan produk ini?
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04Bagaimana cara meminta penawaran harga untuk pembelian dalam jumlah besar, dan apakah COA dapat disediakan?
Ya. Produk ini tersedia dalam 500 g, 5 kg, 25 kg. Sampaikan aplikasi dan volume kebutuhan Anda saat meminta penawaran harga; kami membalas dalam satu hari kerja dan dapat menyediakan COA, lembar spesifikasi, serta lembar data teknis.
Referensi
- 1. Henry D. A., Campbell M. E., LiPuma J. J. and Speert D. P., 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J. Clin. Microbiol., 35:614-619.
- 2. United States Pharmacopeia, current edition. Chapter <60> Microbiological Examination of Nonsterile Products — Tests for Burkholderia cepacia Complex, USP-NF, Rockville, MD.