Gambar hanya ilustrasi. Produk aktual dapat berbeda.ATCC Medium 271
Dikenal juga sebagai：Escherichia Medium · ATCC 271 Medium · E. coli K-12 Medium
For the cultivation of Escherichia coli K-12 strains, as recommended by the American Type Culture Collection.
- Kategori
- Media Kultur Kering
- Larutkan per liter
- 20.3 g
- Ukuran kemasan
- 500 g · 5 kg · 25 kg
Data diperbarui：2026-11-06
Ringkasan
Prinsip
The medium is the formulation recommended by the American Type Culture Collection for the cultivation of Escherichia coli K-12 and its derivatives.¹ Tryptone and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, dextrose provides a readily fermentable carbon and energy source, and sodium chloride maintains the osmotic equilibrium. Calcium chloride supplies calcium ions, and thiamine supports the growth of thiamine-requiring K-12 derivatives commonly used in laboratory work. The heat-sensitive components are supplied separately and added after sterilisation.
Petunjuk penyiapan
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
Respons kultur
| Organisme uji | ATCC | Pertumbuhan |
|---|---|---|
| Escherichia coli K-12 | 10798 | baik-subur |
Keunggulan produk
- Diproduksi sesuai formulasi standar, 20.3 g per liter
- Kinerja diverifikasi terhadap galur kontrol standar sebelum dirilis (Escherichia coli K-12)
- Tersedia dalam 500 g / 5 kg / 25 kg, dari skala laboratorium hingga industri
- Tersedia COA, lembar spesifikasi, dan lembar data teknis; sampel dapat diminta
- Diproduksi di Taiwan dengan pasokan yang andal
Pertanyaan yang sering diajukan
01Berapa gram bubuk per liter?
Gunakan 20.3 g per liter air deionisasi; kondisi penyiapan dan sterilisasi tercantum pada lembar spesifikasi.
02Cocok untuk mikroorganisme apa?
Gram-negative, Aerobes, E. coli, Escherichia coli K-12. Lihat tabel uji kinerja pertumbuhan dan lembar spesifikasi.
03Bagaimana cara menyiapkan produk ini?
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
04Bagaimana cara meminta penawaran harga untuk pembelian dalam jumlah besar, dan apakah COA dapat disediakan?
Ya. Produk ini tersedia dalam 500 g, 5 kg, 25 kg. Sampaikan aplikasi dan volume kebutuhan Anda saat meminta penawaran harga; kami membalas dalam satu hari kerja dan dapat menyediakan COA, lembar spesifikasi, serta lembar data teknis.
Referensi
- 1. American Type Culture Collection, Catalogue of Bacteria and Bacteriophages, ATCC, Manassas, VA.