Reinforced Clostridial Medium w/o Agar
強化梭菌液體培養基(不含洋菜)
別名與縮寫：RCM · Reinforced Clostridial Medium without Agar · RCM without Agar · Reinforced Clostridial Broth · RCB · Reinforced Clostridial Medium (Agar-free) · 強化梭菌培養基 · 厭氧菌培養基
For the cultivation and enrichment of Clostridium species and other anaerobic organisms.
- 類別
- 粉末培養基
- 包裝規格
- 500 g · 5 kg · 25 kg
- 認證
- ISO 22000
資料更新：2026-11-06
- 過敏原
- Milk
產品說明
Reinforced Clostridial Medium w/o Agar 是瓊脂（固態）培養基，供厭氧菌或營養需求較高的菌種使用。營養豐富並含還原劑，用於梭菌與其他厭氧菌的培養與計數。上鼎以食品級原料製造，生產環境通過 ISO 22000 認證，每批附 COA。
原理
Reinforced Clostridial Medium, described by Hirsch and Grinsted, is a nutritionally rich medium for the cultivation, enrichment, and enumeration of clostridia and other anaerobic and facultatively anaerobic organisms.¹ Meat extract, peptone, and yeast extract supply nitrogen, amino acids, vitamins, and the growth factors required by these demanding organisms, allowing growth to be initiated from a small inoculum. Dextrose serves as the carbon and energy source, sodium chloride maintains the osmotic equilibrium, and sodium acetate buffers the medium against the organic acids produced during fermentation. Soluble starch acts as a protective colloid, adsorbing toxic metabolites. L-Cysteine hydrochloride functions as a reducing agent, lowering the redox potential of the medium to the level required for the growth of strict anaerobes.² This formulation contains no agar and is intended for use as a liquid medium for enrichment and propagation. Growth is indicated by turbidity, and gas production by clostridia may be observed as bubbles within the medium.
厭氧菌與挑剔菌培養基
厭氧菌與營養需求較高的菌種需要含有還原劑、血液或浸出液成分的培養基，並配合厭氧培養環境。部分培養基需要在滅菌後添加血液、血清或抗生素等易受熱破壞的成分，添加時機與方式請依配製說明操作。
配製與使用
Suspend 37.5 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool and inoculate as soon as possible after preparation. Incubate under anaerobic conditions. Test the finished medium for performance using stable, standard control cultures.
產品特色
- 食品級原料，生產環境通過 ISO 22000 食品安全管理系統認證
- 500 g / 5 kg / 25 kg 三種以上包裝，研究室到工業規模都適用
- 可提供 COA、規格書與技術資料表，支援樣品申請
- 台灣製造，供貨穩定
食品級與實驗室級的差別
市面上多數培養基供應商使用實驗室級（laboratory grade）原料，在重金屬殘留、微生物污染管控與原料來源追溯上的標準較低，用於純研究足夠，但不適合對原料品質有更高要求的場景。上鼎的產品以食品級（food-grade）原料製造，生產全程在通過 ISO 22000 食品安全管理系統認證的設施內進行。
- 食品微生物檢測實驗室，需符合 ISO 17025 或相關法規要求
- 生技廠商生產供出口的發酵產品，需完整原料溯源文件
- 對供應鏈有 ESG 或合規審查要求的機構
- 同時採購清真與非清真產品、需統一原料等級標準的企業
常見問題
01適用哪些菌種？
厭氧菌、革蘭氏陽性菌、產氣莢膜梭菌、肉毒桿菌、酪酸菌。詳見培養性能表與規格書。
02可以用於食品級用途嗎？
可以。本產品以食品級原料製造，生產環境通過 ISO 22000 食品安全管理系統認證，適用於直接食用的發酵產品的菌種培養。
03這個產品要怎麼配製？
Suspend 37.5 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool and inoculate as soon as possible after preparation. Incubate under anaerobic conditions. Test the finished medium for performance using stable, standard control cultures.
04大量採購如何詢價？可以提供 COA 嗎？
可以。本產品提供 500 g、5 kg、25 kg 等包裝規格，請於詢價時說明應用場景與用量，一個工作天內回覆，並可提供 COA、規格書與技術資料表。
05台灣有現貨嗎？交期多久？
本產品在新北市汐止的自有工廠生產、台灣出貨，不需進口與清關。交期依庫存與包裝規格而定，詢價時告知品項與用量，我們會一併回覆現貨狀況與交期；急件請在詢價單註明。
06可以開立統一發票嗎？學校與研究機構可以請購嗎？
可以。我們開立台灣統一發票（含營業稅），學校、研究機構與公家單位可依請購流程先索取報價單再下單；小包裝與樣品申請都接受。
07有中文版的 SDS 與規格書嗎？
有。規格書（TDS）與 SDS 以英文版為標準文件，登入後可在產品頁下載；中文版 SDS 依需求提供，請在詢價單或聯絡表單註明品項與用途，我們會隨報價寄出。每批出貨附 COA。
參考文獻
- 1. Hirsch A. and Grinsted E., 1954. Methods for the growth and enumeration of anaerobic spore-formers from cheese, with observations on the effect of nisin. J. Dairy Res., 21:101-110.
- 2. Atlas R. M., 2010. Handbook of Microbiological Media, 4th Ed., CRC Press, Boca Raton.