Illustrative image only. Actual product may vary.Tryptone N1
- Category
- Peptones & Protein Hydrolysates
- Pack sizes
- 500 g · 5 kg · 15 kg
- Raw material
- Casein
Data updated：2026-11-06
Overview
Tryptone N1 is a pancreatic digest of casein manufactured by Organotechnie (France) and supplied in Taiwan by STBIO MEDIA. Beyond microbial culture it is widely used in mammalian cell culture as a supplement for serum-free and low-serum media, supplying peptides and growth factors; published work documents its benefit for recombinant protein titres after transfection in HEK293 cells. It suits cell culture, recombinant protein and vaccine processes that demand consistent raw materials.
Choosing between tryptone, casein peptone and casamino acids
All three come from casein; the difference is the hydrolysis. Tryptone is digested with trypsin, giving abundant peptides and high tryptophan, and is the nitrogen source specified in LB, TSB and similar formulations. Casein peptone is the general term for casein hydrolysed with other enzymes, with a peptide profile that varies by product. Casamino acids are acid-hydrolysed, almost entirely free amino acids with no tryptophan, for semi-defined media that need a known composition. When a formulation says tryptone, use tryptone rather than substituting the other two. For cell culture, choose a clear, low-endotoxin grade such as Tryptone N1.
Product highlights
- Available in 500 g / 5 kg / 15 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01What is the difference between tryptone and peptone?
Peptone is the general term for protein hydrolysates; tryptone is one specific kind, made from casein with trypsin, so its peptide profile and tryptophan content are more defined. When a formulation specifies tryptone, do not substitute another peptone directly, as growth and yield may differ.
02Can I make LB medium from tryptone myself?
Yes. Standard LB Miller is 10 g tryptone, 5 g yeast extract and 10 g sodium chloride per litre; Lennox reduces sodium chloride to 5 g. Adjust to pH 7.0 and autoclave. To save preparation time or ensure batch consistency, use our ready-made LB Broth powder instead.
03Why is tryptone used for the indole test?
The indole test checks whether a bacterium can break tryptophan down to indole. Tryptone is high in tryptophan compared with other peptones, so tryptone water or tryptone broth made from it supplies enough substrate for a clear reaction, which is why it is the standard nitrogen source for this test.
04Does tryptone contain allergens? Can it be used in vegetarian or Halal formulations?
Tryptone is derived from milk casein, so it is a milk allergen and must be declared. It contains no meat, but as a dairy derivative it is not vegan; use soy or plant peptones for vegan formulations. For Halal requirements choose the certified version whose code ends in H.
05How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 15 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
- Recombinant protein expression and plasmid production in E. coliLB, TB, and SOC ranges from transformation to high-density culture
- Serum-free and serum-reduced cell culture: cultivated meat, vaccines, and recombinant proteinsPlant- and yeast-derived cell-grade hydrolysates in place of part of the serum
- HEK293 and insect cells: transient expression and viral vectorsHydrolysate supplementation after transfection to raise transient expression
- Vaccine production: serum reduction in Vero cellsPlant peptone supplementation to cut fetal bovine serum use
Used in published research and patents
- Antibodies to interleukin-1beta and uses thereofUS Patent Application 2023/0220065 A1 2023 · Academia Sinica · Published application, not yet granted0.5% Tryptone N1 (ST Bio, Inc.) added to HEK293 cells 24 hours after transfection; supernatant harvested after 6 days for antibody expression.
- Single-chain variant fragment antibody library expressed by phageCN Patent 107614762 B 2022 · Academia Sinica · Granted0.5% Tryptone N1 (STBio, Inc.) added to HEK293 Freestyle cells 24 hours after transfection, with the supernatant harvested after 5 days for antibody expression.
- Recombinant antibody and uses thereofUS Patent 10,472,411 B2 2019 · Academia Sinica · Granted0.5% Tryptone N1 (ST Bio, Inc.) added to HEK293 cells 24 hours after transfection; supernatant harvested after 5 days for recombinant antibody expression.
- Publications
Further reading
- Peptones and Protein Hydrolysates: Applications by IndustryAn overview of where peptones and protein hydrolysates are used: microbial fermentation, mammalian cell culture, clinical and diagnostic media, probiotics, biopharma and vaccines, agrobiotech, and cosmetic fermentation, with guidance on choosing a nitrogen source.
- Optimisation of Protein Production in HEK293 Cells with Different PeptonesOrganotechnie R&D compared plant-derived peptones with Tryptone N1 for IL-4 expression in HEK293 cells, to find peptones that raise recombinant protein titres after transfection.