Tryptone Plus
Tryptone Plus is used as nutrients in many types of fermentation including lactic acid cultures, pharmaceuticals and bioremediation.
- Category
- Peptones & Protein Hydrolysates
- Pack sizes
- 500 g · 5 kg · 25 kg
- Raw material
- Casein
- Certifications
- Non-GMO · Non-TSE/BSE
Data updated：2026-11-06
- Shelf life
- When stored in sealed containers, at ambient temperature in dry and clean conditions, the expected shelf life is 2 years.
Overview
Tryptone Plus is manufactured by a controlled enzymatic hydrolysis of casein. The powder is easily soluble in water and contains a mix of peptides and free amino acids, making it an ideal organic nitrogen source for various applications.
Choosing between tryptone, casein peptone and casamino acids
All three come from casein; the difference is the hydrolysis. Tryptone is digested with trypsin, giving abundant peptides and high tryptophan, and is the nitrogen source specified in LB, TSB and similar formulations. Casein peptone is the general term for casein hydrolysed with other enzymes, with a peptide profile that varies by product. Casamino acids are acid-hydrolysed, almost entirely free amino acids with no tryptophan, for semi-defined media that need a known composition. When a formulation says tryptone, use tryptone rather than substituting the other two. For cell culture, choose a clear, low-endotoxin grade such as Tryptone N1.
Product highlights
- Non-GMO
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01What is the difference between tryptone and peptone?
Peptone is the general term for protein hydrolysates; tryptone is one specific kind, made from casein with trypsin, so its peptide profile and tryptophan content are more defined. When a formulation specifies tryptone, do not substitute another peptone directly, as growth and yield may differ.
02Can I make LB medium from tryptone myself?
Yes. Standard LB Miller is 10 g tryptone, 5 g yeast extract and 10 g sodium chloride per litre; Lennox reduces sodium chloride to 5 g. Adjust to pH 7.0 and autoclave. To save preparation time or ensure batch consistency, use our ready-made LB Broth powder instead.
03Why is tryptone used for the indole test?
The indole test checks whether a bacterium can break tryptophan down to indole. Tryptone is high in tryptophan compared with other peptones, so tryptone water or tryptone broth made from it supplies enough substrate for a clear reaction, which is why it is the standard nitrogen source for this test.
04Does tryptone contain allergens? Can it be used in vegetarian or Halal formulations?
Tryptone is derived from milk casein, so it is a milk allergen and must be declared. It contains no meat, but as a dairy derivative it is not vegan; use soy or plant peptones for vegan formulations. For Halal requirements choose the certified version whose code ends in H.
05Is the raw material non-GMO?
Yes. The raw material is non-GMO; the supplier declaration can be provided with the COA.
06How should it be stored, and what is the shelf life?
When stored in sealed containers, at ambient temperature in dry and clean conditions, the expected shelf life is 2 years.
07How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.