ORGANOTECHNIE · CELL CULTURE GRADE

C-Cell cell-culture-grade protein hydrolysates

Ultrafiltered, low-endotoxin plant and yeast protein hydrolysates made by Organotechnie, France, for serum-free and low-serum culture of CHO, HEK293, Vero, mesenchymal stromal and insect cells. STBIO MEDIA is the distributor in Taiwan, supplying samples, specifications and batch COAs.

Fetal bovine serum is expensive, variable between batches and carries animal-origin risk, so vaccine, monoclonal antibody, cell therapy and cultivated meat processes all look for ways to use less of it. Cell-culture-grade protein hydrolysates are the most common replacement supplement: plant or yeast protein is cut into peptides and amino acids, ultrafiltered to remove large molecules and controlled for endotoxin, so it can supply part of the nutrition and growth factors serum used to provide.

C-Cell is the range Organotechnie designed for cell culture, split by raw material into soy, pea, guar, broad bean, rice, wheat and yeast grades, each with validation data on CHO, HEK293 or Vero. STBIO MEDIA has distributed the range since 2023, holds stock in Taiwan and provides free samples.

Request C-Cell samples Request a quotation Sample trial protocol

UltrafilteredLow endotoxinPlant and yeast derived, animal-origin-freeCOA with endotoxin data for every batchStock in Taiwan, free samplesManufactured by Organotechnie S.A.S., France · Distributed in Taiwan since 2023

Products in the range

C-Cell	Raw material	Use	Validated on
S104 Soy C-Cell S104	Soy	Ultrafiltered, low-endotoxin soy hydrolysate for mammalian cell proliferation.	HEK293, CHO	Product page
S146B Soy C-Cell S146B	Soy	For CHO cell proliferation and protein expression; supports partial replacement of fetal bovine serum.	CHO	Product page
S186 Soy C-Cell S186	Soy	For CHO cell proliferation and protein expression; supports partial replacement of fetal bovine serum.	CHO	Product page
S204 Soy C-Cell S204	Soy	Soy hydrolysate; one of the three best performers in the HEK293 recombinant protein study.	HEK293	Ask for price
P112 Pea C-Cell P112	Pea	Pea hydrolysate for CHO proliferation and protein expression; free of soy and gluten allergens.	CHO, HEK293	Product page
G115 Guar C-Cell G115	Guar	Guar hydrolysate; the manufacturer reports it promotes enzyme protein expression in mammalian cells.	HEK293, CHO	Product page
F120 Broad Bean C-Cell F120	Broad bean	Broad bean hydrolysate for CHO cell proliferation.	CHO	Product page
R116 Rice C-Cell R116	Rice	Rice protein hydrolysate, low allergen, for CHO cell proliferation.	CHO	Product page
R123 Rice C-Cell R123	Rice	Rice protein hydrolysate, low allergen, for CHO cell proliferation.	CHO	Product page
W106 Wheat C-Cell W106	Wheat	Ultrafiltered wheat peptone for cell culture and animal-vaccine microbial fermentation; the best wheat source in the Vero serum-reduction study.	Vero, HEK293	Ask for price
Y109 Yeast C-Cell Y109	Yeast	Ultrafiltered yeast hydrolysate for mammalian and insect cell proliferation and protein expression.	CHO, HEK293, Sf9	Product page
Y121 Yeast C-Cell Y121	Yeast	Ultrafiltered yeast hydrolysate for mammalian and insect cell culture.	CHO, Sf9	Product page

Organotechnie® and C-Cell are trademarks of Organotechnie S.A.S., France. ST BIO, INC. is their distributor in Taiwan; the products are manufactured by Organotechnie.

Cell lines and applications

CHOCHO cells: monoclonal antibodies and recombinant proteinsAdded to serum-free or low-serum basal media to supply peptides and growth factors, supporting cell density and product accumulation. Suspension cultures usually take a higher dose than adherent ones. Most C-Cell grades are validated primarily on CHO.S104S146BS186P112G115F120R116R123Y109Y121
HEK293HEK293 transient expressionAn Organotechnie R&D report expressed IL-4 in HEK293-6E and compared plant-derived C-Cell grades with Tryptone N1; P112, G115 and S204 gave markedly higher expression than the control.S104S204P112G115W106Y109
VeroVero cells and serum reduction for vaccine productionA study by Inserm and Organotechnie achieved the same Vero proliferation with 5% serum plus 0.5 g/L peptone as with 10% serum, halving serum use.W106
MSCMesenchymal stromal cells and extracellular vesicle productionAn Inserm study coated culture surfaces with plant peptone and used EV-depleted fetal bovine serum to raise extracellular vesicle yield from umbilical cord MSCs while retaining function.
Sf9Insect cells and baculovirus expressionYeast-derived Y109 and Y121 are suited to proliferation and protein expression in Sf9 and other insect cells.Y109Y121

Frequently asked questions

01What is C-Cell?

C-Cell is the cell-culture-grade protein hydrolysate range from Organotechnie S.A.S., France: plant or yeast proteins enzymatically hydrolysed and ultrafiltered, with low endotoxin, used to supply peptides and growth factors in serum-free or low-serum media. STBIO MEDIA is the distributor in Taiwan.

02How does C-Cell differ from an ordinary peptone?

Three things: ultrafiltration (removing large molecules and potential viral or endotoxin load), an endotoxin specification, and performance testing on cell lines. Ordinary fermentation peptones lack these and often inhibit growth or introduce endotoxin when added to cell culture media.

03What dose should I start with?

It varies widely with the cell type and culture mode. In published studies, adherent Vero cells used 0.5 g per litre (0.05%) while suspension HEK293 used 0.5% (5 g per litre), a tenfold difference. Trial three levels between 0.25 and 5 g per litre, starting low for adherent cells and high for suspension cells, then read cell density and product.

04Can it fully replace serum?

It depends on the cell line. Published data show serum can be cut from 10% to 5% in Vero and partially replaced in CHO; fully serum-free culture usually needs an optimised basal medium and adaptation. We supply samples so you can verify in your own system.

05What documentation is available?

Each batch comes with a COA (including endotoxin), a TDS and an SDS; the manufacturer can provide animal-origin, non-GMO and process statements. Contact us for documents required for regulatory filings.

06Is it animal-origin-free?

Plant- and yeast-derived C-Cell grades contain no animal-derived raw material; the origin of enzymes and processing aids is stated in the manufacturer's declaration, available with the sample.

07Minimum order and lead time?

Samples are free; order sizes follow the pack formats. Common grades are stocked in Taiwan and the rest ship from France. Tell us the cell line, application and volume via the quotation form.

Research reports

The following are summaries, written in our own words, of research reports published independently by their authors and institutions. Results belong to the original reports and are not product claims by STBIO MEDIA.

Serum reduction estimate

Enter your own volumes and prices to see the annual difference when you cut serum and add C-Cell. Every number is yours; this site assumes no prices.

Medium used per year (litres)C-Cell dose (%, w/v)Current serum level (%)Target serum level (%)Serum price (per litre)C-Cell price (per kg, ask sales)

Serum saved per year: 50 L
C-Cell needed: 0.5 kg
Serum cost saved: —
C-Cell cost: —
Net annual difference: —

Enter serum and C-Cell prices to see amounts. An estimate only; excludes medium, labour and validation costs. Whether serum can be reduced must be confirmed on your own cell line. Amounts are in the currency you entered.

Validate with your own cell lineTell us the cell line, basal medium and current serum level, and we will send three or four C-Cell samples with a suggested trial design.

Sample trial protocol Request C-Cell samples