Tryptone
Tryptone is used as a nutrient source in various types of fermentation including lactic acid cultures, pharmaceutical production and bioremediation.
- Category
- Peptones & Protein Hydrolysates
- Pack sizes
- 500 g · 5 kg · 25 kg
- Raw material
- Casein
- Certifications
- ISO 22000 · Halal
Data updated：2026-11-06
- Allergens
- Milk
Overview
Tryptone is a fine beige powder, produced by controlled enzymatic hydrolysis of casein. It contains a high level of protein, in readily absorbable pre-digested forms such as peptides and amino acids, soluble vitamins, minerals and other growth factors.
Choosing between tryptone, casein peptone and casamino acids
All three come from casein; the difference is the hydrolysis. Tryptone is digested with trypsin, giving abundant peptides and high tryptophan, and is the nitrogen source specified in LB, TSB and similar formulations. Casein peptone is the general term for casein hydrolysed with other enzymes, with a peptide profile that varies by product. Casamino acids are acid-hydrolysed, almost entirely free amino acids with no tryptophan, for semi-defined media that need a known composition. When a formulation says tryptone, use tryptone rather than substituting the other two. For cell culture, choose a clear, low-endotoxin grade such as Tryptone N1.
Product highlights
- Food-grade raw materials, produced in an ISO 22000 certified facility
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Food grade versus laboratory grade
Most culture media suppliers use laboratory-grade raw materials, which are held to lower standards for heavy metal residues, microbial contamination control, and traceability. That is adequate for pure research but not for applications with stricter raw-material requirements. STBIO manufactures with food-grade raw materials throughout, in a facility certified to ISO 22000 food safety management.
- Food microbiology laboratories working to ISO 17025 or equivalent requirements
- Biotech manufacturers exporting fermentation products and needing full raw-material traceability
- Organisations with ESG or compliance audit requirements across their supply chain
- Companies sourcing both Halal and non-Halal products who need one consistent material grade
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 4: Enumeration of Escherichia coli and the Coliform BacteriaBAM Chapter 4Confirmation / biochemicalNamed in the method as：Tryptone (tryptophane) broth, indole test
- FDA BAM Chapter 5: SalmonellaBAM Chapter 5Confirmation / biochemicalNamed in the method as：Tryptone (tryptophane) broth
- FDA BAM Chapter 6: ShigellaBAM Chapter 6Confirmation / biochemicalNamed in the method as：Tryptone broth, indole test
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria（BAM Chapter 4）: Confirmation / biochemical; FDA BAM Chapter 5: Salmonella（BAM Chapter 5）: Confirmation / biochemical; FDA BAM Chapter 6: Shigella（BAM Chapter 6）: Confirmation / biochemical. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02What is the difference between tryptone and peptone?
Peptone is the general term for protein hydrolysates; tryptone is one specific kind, made from casein with trypsin, so its peptide profile and tryptophan content are more defined. When a formulation specifies tryptone, do not substitute another peptone directly, as growth and yield may differ.
03Can I make LB medium from tryptone myself?
Yes. Standard LB Miller is 10 g tryptone, 5 g yeast extract and 10 g sodium chloride per litre; Lennox reduces sodium chloride to 5 g. Adjust to pH 7.0 and autoclave. To save preparation time or ensure batch consistency, use our ready-made LB Broth powder instead.
04Why is tryptone used for the indole test?
The indole test checks whether a bacterium can break tryptophan down to indole. Tryptone is high in tryptophan compared with other peptones, so tryptone water or tryptone broth made from it supplies enough substrate for a clear reaction, which is why it is the standard nitrogen source for this test.
05Does tryptone contain allergens? Can it be used in vegetarian or Halal formulations?
Tryptone is derived from milk casein, so it is a milk allergen and must be declared. It contains no meat, but as a dairy derivative it is not vegan; use soy or plant peptones for vegan formulations. For Halal requirements choose the certified version whose code ends in H.
06Which organisms is it suitable for?
Lactic acid bacteria. See the cultural response table and the specification sheet.
07Can it be used for food-grade applications?
Yes. It is made from food-grade raw materials in an ISO 22000 certified facility and is suitable for culturing starters for fermented products that are consumed directly.
08Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
09How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
- Recombinant protein expression and plasmid production in E. coliLB, TB, and SOC ranges from transformation to high-density culture
- High Cell Density Fermentation Nutrient SeriesBalanced, efficient nutrients that support high-cell-density cultivation, increasing cell concentration, biomass and volumetric productivity. For E. coli, Bacillus and other industrial fermentation processes.
- Recombinant Protein Expression Nutrient SeriesAmino acids, peptides and growth factors that support recombinant protein expression and soluble protein yield. For recombinant E. coli, vaccine antigens, industrial enzymes and biopharmaceutical production.
Used in published research and patents
- Aspergillus oryzae Fermentation Extract Alleviates Inflammation in Mycoplasma pneumoniae PneumoniaMolecules 2023 · National Taiwan UniversityPPLO Broth and pancreatic digest of casein used in the Mycoplasma pneumoniae culture medium.
- Publications
Further reading
- FDA BAM Culture Media Cross-Reference: Which Media Each BAM Chapter Uses and the STBIO EquivalentAerobic plate count, coliforms and E. coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Vibrio, Clostridium perfringens, yeasts and moulds and Cronobacter: the media named in each FDA Bacteriological Analytical Manual chapter, matched to Taiwan-made dehydrated media for US laboratories qualifying a second source.
- Which Culture Media Do Taiwan FDA Food Microbiology Methods Specify? Cross-Reference for 21 Announced MethodsAerobic plate count, coliforms, E. coli, Enterobacteriaceae, Salmonella, Staphylococcus aureus, Listeria monocytogenes, lactic acid bacteria, yeasts and moulds, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens and Cronobacter: the media named in each Taiwan FDA (TFDA) announced method, matched to Taiwan-made products.
- What Are Casamino Acids? Casamino Acids vs Tryptone vs Casein PeptoneCasamino acids are casein hydrolysed completely with hydrochloric acid, consisting almost entirely of free amino acids. How they differ from tryptone and enzymatic casein peptone in hydrolysis, tryptophan, salt and use.