Imej ilustrasi sahaja. Produk sebenar mungkin berbeza.Burkholderia Cepacia Selective Agar Base
Juga dikenali sebagai：BCSA Base · Burkholderia Cepacia Agar Base · BCSA · Burkholderia cepacia Selective Agar
For the selective isolation of Burkholderia cepacia complex from non-sterile pharmaceutical products and other materials, following the addition of a selective supplement.
- Kategori
- Media Kultur Kering
- Larutkan per liter
- 50.58 g
- Saiz pek
- 500 g · 5 kg · 25 kg
Data dikemas kini：2026-11-13
Gambaran keseluruhan
Prinsip
Burkholderia Cepacia Selective Agar was developed by Henry and co-workers as an improvement on earlier selective media, giving faster growth and better selectivity for the Burkholderia cepacia complex.¹ Pancreatic digest of casein and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Most strains of the Burkholderia cepacia complex oxidise these sugars slowly and release alkaline end-products from the peptones, so that colonies and the surrounding medium turn pink to red after prolonged incubation. Selectivity is provided by crystal violet, which inhibits Gram-positive organisms, vancomycin, which inhibits enterococci and other Gram-positive organisms not suppressed by crystal violet, and polymyxin B with gentamicin, which act together against other aerobic Gram-negative bacilli including Pseudomonas. The medium is specified in the pharmacopoeia for the examination of non-sterile products for the Burkholderia cepacia complex.²
Cara penyediaan
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Tindak balas kultur
| Organisma ujian | ATCC | Pertumbuhan |
|---|---|---|
| Burkholderia cepacia | 25416 | baik-subur |
| Pseudomonas aeruginosa | 9027 | direncat |
| Escherichia coli | 8739 | direncat |
| Staphylococcus aureus | 6538 | direncat |
Kelebihan produk
- Dikilangkan mengikut formulasi standard, 50.58 g bagi setiap liter
- Prestasi disahkan terhadap strain kawalan standard sebelum pelepasan (Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus)
- Tersedia dalam 500 g / 5 kg / 25 kg, daripada skala makmal hingga industri
- COA, helaian spesifikasi dan helaian data teknikal tersedia; sampel atas permintaan
- Dikilangkan di Taiwan dengan bekalan yang boleh dipercayai
Soalan lazim
01Berapa banyak serbuk bagi setiap liter?
Gunakan 50.58 g bagi setiap liter air ternyahion; keadaan penyediaan dan pensterilan dinyatakan pada helaian spesifikasi.
02Organisma manakah yang sesuai?
Gram-negative, Pathogens, Aerobes, Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus. Lihat jadual tindak balas kultur dan helaian spesifikasi.
03Bagaimana produk ini disediakan?
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04Bagaimana saya meminta sebut harga pukal, dan bolehkah anda menyediakan COA?
Ya. Produk ini tersedia dalam 500 g, 5 kg, 25 kg. Beritahu kami aplikasi dan isi padu anda semasa meminta sebut harga; kami membalas dalam satu hari bekerja dan boleh membekalkan COA, helaian spesifikasi dan helaian data teknikal.
Rujukan
- 1. Henry D. A., Campbell M. E., LiPuma J. J. and Speert D. P., 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J. Clin. Microbiol., 35:614-619.
- 2. United States Pharmacopeia, current edition. Chapter <60> Microbiological Examination of Nonsterile Products — Tests for Burkholderia cepacia Complex, USP-NF, Rockville, MD.