Illustrative image only. Actual product may vary.Yeast C-Cell Y109
Designed for cell culture; supports the proliferation and protein expression of mammalian and insect cells.
- Category
- Peptones & Protein Hydrolysates
- pH (25 °C)
- 5.2 – 5.8
- Appearance
- Pale-yellow powder
- Pack sizes
- 500 g · 5 kg · 25 kg
- Raw material
- Yeast
Data updated:2026-11-06
- Storage
- Store at 10–30 °C in sealed, dry, clean conditions. Hygroscopic: keep the container tightly closed when not in use.
- Shelf life
- 3 years
Overview
Ultrafiltered yeast extract obtained by autolysis of Saccharomyces cerevisiae; low in endotoxin. Fine pale-yellow powder, readily soluble in water, containing peptides, free amino acids, purine and pyrimidine bases and water-soluble B-group vitamins.
Specifications
| Parameter | Specification |
|---|---|
| Appearance | Pale-yellow powder |
| Solubility (5 % solution) | Complete |
| pH (5 % solution) | 5.2 – 5.8 |
| Loss on drying | ≤ 6.0 % |
| Total nitrogen (TN) | 10.0 – 10.7 % |
| Amino nitrogen (AN) | 4.9 – 5.6 % |
| AN/TN × 100 | 46 – 56 |
| Residue on ignition | ≤ 12.0 % |
| Chlorides (as NaCl) | ≤ 1.0 % |
| Filterability (100 mL, 5 % w/v, 0.22 µm) | ≤ 300 sec |
| Endotoxin | ≤ 200 EU/g |
| Total aerobic microbial count | ≤ 1,000/g |
| Staphylococcus aureus | absence/10g |
| Salmonella | absence/25g |
| Escherichia coli | absence/g |
| Coliforms | ≤ 10/g |
| Yeasts and moulds | ≤ 20/g |
Release specifications. Actual values are reported on the COA supplied with each batch.
Product highlights
- Ultrafiltered (UF), Low endotoxin
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Shipped from Taiwan with reliable supply
Frequently asked questions
01Which cell lines is this C-Cell grade validated on?
Used on CHO, HEK293, Sf9 in manufacturer data and research reports. Ultrafiltered yeast hydrolysate for mammalian and insect cell proliferation and protein expression. For other cell lines, start with a sample trial.
02What dose, and how is it added to the medium?
It varies with the cell type and culture mode: published studies used 0.5 g per litre for adherent Vero and 5 g per litre for suspension HEK293. Prepare a 30 g per litre stock directly in culture medium, filter through 0.2 µm and dilute in; trial three levels between 0.25 and 5 g per litre and read cell density and product.
03How should it be stored, and what is the shelf life?
Store at 10–30 °C in sealed, dry, clean conditions. Hygroscopic: keep the container tightly closed when not in use. 3 years
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
- CHO cells: monoclonal antibody and recombinant protein productionPeptide supplementation in serum-free CHO processes to support cell density and protein expression
- Serum-free and serum-reduced cell culture: cultivated meat, vaccines, and recombinant proteinsPlant- and yeast-derived cell-grade hydrolysates in place of part of the serum
- HEK293 and insect cells: transient expression and viral vectorsHydrolysate supplementation after transfection to raise transient expression
- Vaccine production: serum reduction in Vero cellsPlant peptone supplementation to cut fetal bovine serum use
