Illustrative image only. Actual product may vary.Tryptic Soy Broth
Also known as：TSB
Used for the propagation and cultivation of Saccharomyces cerevisiae and other yeasts molecular microbiology procedures.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 30 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Standard methods
- USP <61> / <62>·EP 2.6.12 / 2.6.13·ISO 11133
Data updated：2026-11-06
Brand cross-reference：BD Difco Tryptic Soy Broth (BBL Trypticase Soy Broth) · Oxoid Tryptone Soya Broth (TSB) · Merck CASO Broth（USP <61> / <62>、EP 2.6.12 / 2.6.13、ISO 11133） Brand cross-reference
Overview
Tryptic Soy Broth (TSB) is the most widely used general-purpose liquid medium: tryptone and soy peptone supply abundant peptides and amino acids, glucose is the carbon source and phosphate buffers the pH. Both fastidious and non-fastidious bacteria grow in it, so it serves routine culture, enrichment, seed culture, sterility testing and environmental monitoring, and is one of the media specified in pharmacopoeial microbial limit tests, making it the highest-volume liquid medium in pharmaceutical, food and biotech laboratories.
Choosing a general-purpose or enumeration medium
Plate Count Agar is used for total viable counts in food, water, and environmental samples. Nutrient Broth and Tryptic Soy media are the general-purpose choices for non-fastidious bacteria, with the latter richer and also used for sterility testing and environmental monitoring. Brain Heart Infusion is the richest of the three and suits fastidious organisms such as streptococci. Use the agar form for enumeration and the broth for propagation.
Product highlights
- Manufactured to the standard formulation, 30 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 5: SalmonellaBAM Chapter 5Confirmation / biochemicalNamed in the method as：Trypticase (tryptic) soy broth
- FDA BAM Chapter 9: VibrioBAM Chapter 9Base mediumNamed in the method as：Trypticase soy broth with 2% or 3% NaCl (base)
- FDA BAM Chapter 10: Listeria monocytogenesBAM Chapter 10Confirmation / biochemicalNamed in the method as：Trypticase soy broth with 0.6% yeast extract (TSBYE)
- FDA BAM Chapter 12: Staphylococcus aureusBAM Chapter 12Base mediumNamed in the method as：Trypticase soy broth with 10% NaCl and 1% sodium pyruvate (base)
- FDA BAM Chapter 14: Bacillus cereusBAM Chapter 14Base mediumNamed in the method as：Trypticase soy-polymyxin broth (base)
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 5: Salmonella（BAM Chapter 5）: Confirmation / biochemical; FDA BAM Chapter 9: Vibrio（BAM Chapter 9）: Base medium; FDA BAM Chapter 10: Listeria monocytogenes（BAM Chapter 10）: Confirmation / biochemical; FDA BAM Chapter 12: Staphylococcus aureus（BAM Chapter 12）: Base medium; FDA BAM Chapter 14: Bacillus cereus（BAM Chapter 14）: Base medium. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02Which medium should I use for total viable counts?
Total viable counts in food and water normally use Plate Count Agar with the pour-plate method at 30 °C. Follow the incubation conditions specified by the method you are working to.
03How much powder per litre?
Use 30 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
04What are the equivalent brand products and standard methods?
Cross-reference: BD Difco Tryptic Soy Broth (BBL Trypticase Soy Broth), Oxoid Tryptone Soya Broth (TSB), Merck CASO Broth. Standard methods: USP <61> / <62>, EP 2.6.12 / 2.6.13, ISO 11133. Formulations may differ slightly between makers; refer to the specification sheet.
05Is there a Halal certified version of this product?
Yes. This is the standard version; a Halal certified version is also produced from food-grade raw materials with Halal documentation, for compliant sourcing in Malaysia, Indonesia, and the Middle East.
06How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paperUsed in these applications
- Sterility testingThioglycollate and Tryptic Soy media for pharmacopoeial sterility testing
- Fill media for beverage and food pipeline cleaning verificationAfter CIP, confirm with liquid media that the line is actually clean
- Semiconductor wastewater biotreatment: media and nitrogen sources for nitrifiers, TMAH degraders and sludge acclimationStandard media and industrial nitrogen sources for culturing bioaugmentation strains and starting or re-seeding biological treatment tanks
Used in published research and patents
- Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulationAnimal Microbiome 2024 · National Taiwan UniversityTryptic Soy Broth used for Nocardia seriolae, MRS medium for lactobacilli and LB broth for Bacillus subtilis natto.
- Publications
Further reading
- FDA BAM Culture Media Cross-Reference: Which Media Each BAM Chapter Uses and the STBIO EquivalentAerobic plate count, coliforms and E. coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Vibrio, Clostridium perfringens, yeasts and moulds and Cronobacter: the media named in each FDA Bacteriological Analytical Manual chapter, matched to Taiwan-made dehydrated media for US laboratories qualifying a second source.
- Which Culture Media Do Taiwan FDA Food Microbiology Methods Specify? Cross-Reference for 21 Announced MethodsAerobic plate count, coliforms, E. coli, Enterobacteriaceae, Salmonella, Staphylococcus aureus, Listeria monocytogenes, lactic acid bacteria, yeasts and moulds, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens and Cronobacter: the media named in each Taiwan FDA (TFDA) announced method, matched to Taiwan-made products.
- Biological Treatment of Semiconductor Wastewater: Ammonia, TMAH and the Role of Culture MediaWhy fabs and panel plants grow bacteria: ammonia is removed by nitrification and denitrification, TMAH by dedicated degraders and organics by activated sludge. How to choose media and nitrogen sources for culturing bioaugmentation strains and acclimating or re-seeding biological tanks.