Tryptic Soy Broth H
Also known as：TSB · Soybean-Casein Digest Medium · Casein Soya Bean Digest Broth · Trypticase Soy Broth · SCDM · Soybean Casein Digest Broth
Tryptic Soy Broth H is a general-purpose Halal certified medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 30 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
- Standard methods
- USP <61> / <62>·EP 2.6.12 / 2.6.13·ISO 11133
Data updated：2026-11-06
Brand cross-reference：BD Difco Tryptic Soy Broth (BBL Trypticase Soy Broth) · Oxoid Tryptone Soya Broth (TSB) · Merck CASO Broth（USP <61> / <62>、EP 2.6.12 / 2.6.13、ISO 11133） Brand cross-reference
Overview
Tryptic Soy Broth H is the Halal-certified version of TSB, identical in formulation and use: tryptone and soy peptone supply nitrogen, glucose the carbon source, and phosphate buffers the pH, supporting fastidious and non-fastidious bacteria alike. It serves routine culture, enrichment, sterility testing and environmental monitoring, with growth performance verified against reference strains before release. Made from food-grade raw materials with Halal documentation for pharmaceutical, food and contract customers who need it.
Principle
Tryptic Soy Broth H, described in the pharmacopoeias as Soybean-Casein Digest Medium, is a general-purpose medium that supports the growth of a wide range of aerobic and facultatively anaerobic organisms, including many fastidious species.¹ Pancreatic digest of casein and papaic digest of soybean meal together provide nitrogen, amino acids, peptides, and the natural growth factors required by fastidious organisms; the soybean digest is particularly rich in carbohydrates. Sodium chloride maintains the osmotic equilibrium of the medium, dipotassium hydrogen phosphate acts as a buffer to limit pH shifts during growth, and dextrose serves as the carbon and energy source. The absence of selective or inhibitory agents makes the medium suitable for sterility testing, microbial limit testing, environmental monitoring, and the general propagation and maintenance of cultures.²
Choosing a general-purpose or enumeration medium
Plate Count Agar is used for total viable counts in food, water, and environmental samples. Nutrient Broth and Tryptic Soy media are the general-purpose choices for non-fastidious bacteria, with the latter richer and also used for sterility testing and environmental monitoring. Brain Heart Infusion is the richest of the three and suits fastidious organisms such as streptococci. Use the agar form for enumeration and the broth for propagation.
Directions
Suspend 30 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating to prevent caramelization of the dextrose. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Neisseria meningitidis | 13090 | Fair to good |
| Staphylococcus epidermidis | 12228 | Good |
| Streptococcus pneumoniae | 6305 | Good |
| Streptococcus pyogenes | 19615 | Good |
| Aspergillus brasiliensis (niger) | 16404 | Growth (30-35°C) |
| Aspergillus brasiliensis (niger) | 16404 | Growth (20-25°C) |
| Bacillus subtilis | 6633 | Growth (30-35°C) |
| Bacillus subtilis | 6633 | Growth (20-25°C) |
| Candida albicans | 10231 | Growth (30-35°C) |
| Candida albicans | 10231 | Growth (20-25°C) |
| Escherichia coli | 8739 | Growth |
| Pseudomonas aeruginosa | 9027 | Growth |
| Salmonella enterica subsp. enterica serotype Typhimurium | 14028 | Growth |
| Staphylococcus aureus | 6538 | Growth |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 30 g per litre
- Performance verified against standard control strains before release (Neisseria meningitidis, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Aspergillus brasiliensis (niger), Aspergillus brasiliensis (niger), Bacillus subtilis, Bacillus subtilis, Candida albicans, Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Typhimurium, Staphylococcus aureus)
- Non-GMO
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 5: SalmonellaBAM Chapter 5Confirmation / biochemicalNamed in the method as：Trypticase (tryptic) soy broth
- FDA BAM Chapter 9: VibrioBAM Chapter 9Base mediumNamed in the method as：Trypticase soy broth with 2% or 3% NaCl (base)
- FDA BAM Chapter 10: Listeria monocytogenesBAM Chapter 10Confirmation / biochemicalNamed in the method as：Trypticase soy broth with 0.6% yeast extract (TSBYE)
- FDA BAM Chapter 12: Staphylococcus aureusBAM Chapter 12Base mediumNamed in the method as：Trypticase soy broth with 10% NaCl and 1% sodium pyruvate (base)
- FDA BAM Chapter 14: Bacillus cereusBAM Chapter 14Base mediumNamed in the method as：Trypticase soy-polymyxin broth (base)
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 5: Salmonella（BAM Chapter 5）: Confirmation / biochemical; FDA BAM Chapter 9: Vibrio（BAM Chapter 9）: Base medium; FDA BAM Chapter 10: Listeria monocytogenes（BAM Chapter 10）: Confirmation / biochemical; FDA BAM Chapter 12: Staphylococcus aureus（BAM Chapter 12）: Base medium; FDA BAM Chapter 14: Bacillus cereus（BAM Chapter 14）: Base medium. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02Which medium should I use for total viable counts?
Total viable counts in food and water normally use Plate Count Agar with the pour-plate method at 30 °C. Follow the incubation conditions specified by the method you are working to.
03How much powder per litre?
Use 30 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
04Which organisms is it suitable for?
Total Plate Count, Aerobes, Environmental monitoring, Neisseria meningitidis, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Aspergillus brasiliensis (niger), Bacillus subtilis, Candida albicans, Escherichia coli, Pseudomonas aeruginosa. See the cultural response table and the specification sheet.
05What are the equivalent brand products and standard methods?
Cross-reference: BD Difco Tryptic Soy Broth (BBL Trypticase Soy Broth), Oxoid Tryptone Soya Broth (TSB), Merck CASO Broth. Standard methods: USP <61> / <62>, EP 2.6.12 / 2.6.13, ISO 11133. Formulations may differ slightly between makers; refer to the specification sheet.
06Is the raw material non-GMO?
Yes. The raw material is non-GMO; the supplier declaration can be provided with the COA.
07How is this product prepared?
Suspend 30 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating to prevent caramelization of the dextrose. Test the finished medium for performance using stable, standard control cultures.
08Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
09How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paperUsed in these applications
- Sterility testingThioglycollate and Tryptic Soy media for pharmacopoeial sterility testing
- Cleanroom and production environment monitoringMedia for settle plates, contact plates, and surface sampling
- Fill media for beverage and food pipeline cleaning verificationAfter CIP, confirm with liquid media that the line is actually clean
- Total plate countRoutine QC of food, drinking water, and environmental samples
Used in published research and patents
- Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulationAnimal Microbiome 2024 · National Taiwan UniversityTryptic Soy Broth used for Nocardia seriolae, MRS medium for lactobacilli and LB broth for Bacillus subtilis natto.
- Publications
References
- 1. United States Pharmacopeia, current edition. Soybean–Casein Digest Medium, USP-NF, Rockville, MD.
- 2. MacFaddin J. F., 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Williams & Wilkins, Baltimore.