Cell therapy: mesenchymal stromal cells and extracellular vesicles
Plant peptone surface coating to raise extracellular vesicle yield
THE SITUATION AND THE PAIN POINTS
Mesenchymal stromal cells (MSCs) and the extracellular vesicles (EVs) they secrete are a major direction in cell and acellular therapy. EV production is often run under starvation or in chemically defined media, which lowers viability and limits yield; ordinary serum adds its own vesicles and compromises purity.
OUR APPROACH
A public Inserm study coated culture surfaces with plant peptone and used EV-depleted fetal bovine serum to raise EV yield from umbilical cord MSCs, with function retained in wound-healing and migration assays. We supply plant-derived C-Cell cell-culture hydrolysates for testing coating and medium supplementation conditions.
- Published study: plant peptone coating raised EV yield
- Plant-derived, introducing no animal-derived vesicles
- Ultrafiltered and low in endotoxin for downstream purification and analysis
- Samples and a trial protocol available
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Frequently asked questions
01How is the coating done?
Follow the original report for details. A typical approach covers the culture surface with a peptone solution, leaves it to stand, removes it and then seeds the cells; concentration and time must be optimised in your system.
02Will it interfere with EV analysis?
Plant peptone carries no animal-derived vesicles, and ultrafiltered products are mainly small molecules that tangential flow filtration separates from EVs. Run a control to confirm.