Imej ilustrasi sahaja. Produk sebenar mungkin berbeza.ATCC Medium 271
Juga dikenali sebagai：Escherichia Medium · ATCC 271 Medium · E. coli K-12 Medium
For the cultivation of Escherichia coli K-12 strains, as recommended by the American Type Culture Collection.
- Kategori
- Media Kultur Kering
- Larutkan per liter
- 20.3 g
- Saiz pek
- 500 g · 5 kg · 25 kg
Data dikemas kini：2026-11-06
Gambaran keseluruhan
Prinsip
The medium is the formulation recommended by the American Type Culture Collection for the cultivation of Escherichia coli K-12 and its derivatives.¹ Tryptone and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, dextrose provides a readily fermentable carbon and energy source, and sodium chloride maintains the osmotic equilibrium. Calcium chloride supplies calcium ions, and thiamine supports the growth of thiamine-requiring K-12 derivatives commonly used in laboratory work. The heat-sensitive components are supplied separately and added after sterilisation.
Cara penyediaan
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
Tindak balas kultur
| Organisma ujian | ATCC | Pertumbuhan |
|---|---|---|
| Escherichia coli K-12 | 10798 | baik-subur |
Kelebihan produk
- Dikilangkan mengikut formulasi standard, 20.3 g bagi setiap liter
- Prestasi disahkan terhadap strain kawalan standard sebelum pelepasan (Escherichia coli K-12)
- Tersedia dalam 500 g / 5 kg / 25 kg, daripada skala makmal hingga industri
- COA, helaian spesifikasi dan helaian data teknikal tersedia; sampel atas permintaan
- Dikilangkan di Taiwan dengan bekalan yang boleh dipercayai
Soalan lazim
01Berapa banyak serbuk bagi setiap liter?
Gunakan 20.3 g bagi setiap liter air ternyahion; keadaan penyediaan dan pensterilan dinyatakan pada helaian spesifikasi.
02Organisma manakah yang sesuai?
Gram-negative, Aerobes, E. coli, Escherichia coli K-12. Lihat jadual tindak balas kultur dan helaian spesifikasi.
03Bagaimana produk ini disediakan?
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
04Bagaimana saya meminta sebut harga pukal, dan bolehkah anda menyediakan COA?
Ya. Produk ini tersedia dalam 500 g, 5 kg, 25 kg. Beritahu kami aplikasi dan isi padu anda semasa meminta sebut harga; kami membalas dalam satu hari bekerja dan boleh membekalkan COA, helaian spesifikasi dan helaian data teknikal.
Rujukan
- 1. American Type Culture Collection, Catalogue of Bacteria and Bacteriophages, ATCC, Manassas, VA.