Tryptic Soy Broth with Bromocresol Purple
Also known as：TSB-BCP · TSB with Bromocresol Purple · Soybean Casein Digest Broth with BCP · Modified TSB with Bromcresol Purple
For the cultivation of biological indicator organisms and the detection of acid production following sterilization processes.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 30.02 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
Overview
Tryptic Soy Broth with Bromocresol Purple is a broth (liquid) medium, a standard general-purpose and enumeration formulation. It is used for enrichment of aerobes and fungi, sterility testing and environmental monitoring. STBIO manufactures it from food-grade raw materials in an ISO 22000 certified facility, with a COA for every batch. This is the Halal-certified version.
Principle
Tryptic Soy Broth, also known as Soybean-Casein Digest Medium, is a general-purpose medium that supports the growth of a wide range of microorganisms.¹ʼ² Pancreatic digest of casein and papaic digest of soybean meal provide nitrogen, amino acids, and peptides for growth.¹ʼ² Sodium chloride maintains the osmotic balance, dipotassium phosphate buffers the medium, and dextrose serves as a fermentable carbohydrate. Bromocresol purple is incorporated as a pH indicator. When organisms grow and ferment the dextrose, the organic acids produced lower the pH and change the indicator from purple to yellow, providing a visual indication of acid production.
Choosing a general-purpose or enumeration medium
Plate Count Agar is used for total viable counts in food, water, and environmental samples. Nutrient Broth and Tryptic Soy media are the general-purpose choices for non-fastidious bacteria, with the latter richer and also used for sterility testing and environmental monitoring. Brain Heart Infusion is the richest of the three and suits fastidious organisms such as streptococci. Use the agar form for enumeration and the broth for propagation.
Directions
Suspend 30.02 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating to prevent caramelization of the dextrose. Test the finished medium for performance using stable, standard control cultures.
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 30.02 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01Which medium should I use for total viable counts?
Total viable counts in food and water normally use Plate Count Agar with the pour-plate method at 30 °C. Follow the incubation conditions specified by the method you are working to.
02How much powder per litre?
Use 30.02 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03How is this product prepared?
Suspend 30.02 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating to prevent caramelization of the dextrose. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paperUsed in these applications
References
- 1. MacFaddin J. F., 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Williams & Wilkins, Baltimore.
- 2. United States Pharmacopeia, current edition. Soybean–Casein Digest Medium, USP-NF, Rockville, MD.