ISP Broth 6
Also known as：ISP6 · ISP Medium No. 6 · ISP-6 · Peptone-Yeast Extract Iron Agar · Peptone Yeast Extract Iron Agar · ISP6 Broth
ISP Broth 6 is used for isolating Streptomyces species according to the International Streptomyces Project.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 22.58 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
Overview
ISP Broth 6 is a broth (liquid) medium, part of the International Streptomyces Project (ISP) set for actinomycete taxonomy. It is ISP 6 peptone-yeast extract-iron formulation, used to read melanin production by Streptomyces. STBIO manufactures it from food-grade raw materials in an ISO 22000 certified facility, with a COA for every batch. This is the Halal-certified version.
Principle
The medium contains peptone, proteose peptone, and yeast extract as sources of carbon, nitrogen, sulfur, and B-complex vitamins. Dipotassium hydrogen phosphate provides buffering capacity to maintain a stable pH. Ferric ammonium citrate and sodium thiosulfate act as the indicator system for the detection of hydrogen sulfide (H₂S) production.
The ISP range for actinomycetes
The ISP (International Streptomyces Project) range is the standard media set for the taxonomy of Streptomyces and other actinomycetes. Each number provides a different carbon and nitrogen combination for observing mycelial colour, soluble pigment, and sporulation. Taxonomic descriptions normally require several of the numbered media read together.
Directions
Suspend 22.6 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121 °C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Streptomyces albus | 3004 | Good |
| Streptomyces lavendulae | 8664 | Good |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 22.58 g per litre
- Performance verified against standard control strains before release (Streptomyces albus, Streptomyces lavendulae)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 22.58 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Streptomyces albus, Streptomyces lavendulae. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 22.6 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121 °C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.