Casamino Acids
Also known as：Casein Hydrolysate · Hydrolysed Casein · Casein Amino Acids
Used as a nutrient source in a wide range of fermentation processes, including lactic acid cultures, pharmaceutical production, and bioremediation.
- Category
- Peptones & Protein Hydrolysates
- pH (25 °C)
- 6.5 – 7.5
- Appearance
- Beige powder
- Pack sizes
- 500 g · 5 kg · 25 kg
- Raw material
- Casein
- Certifications
- ISO 22000 · Halal · Non-GMO · Non-TSE/BSE
Data updated：2026-11-06
- Shelf life
- When stored in sealed containers, at ambient temperature in dry and clean conditions, the expected shelf life is 2 years.
- Allergens
- Milk
Overview
A fine beige, water-soluble powder produced by controlled enzymatic hydrolysis of casein. Rich in readily assimilable peptides and free amino acids, it also supplies soluble vitamins, minerals, and other growth factors. Raw materials are of animal (milk) origin.
Choosing between tryptone, casein peptone and casamino acids
All three come from casein; the difference is the hydrolysis. Tryptone is digested with trypsin, giving abundant peptides and high tryptophan, and is the nitrogen source specified in LB, TSB and similar formulations. Casein peptone is the general term for casein hydrolysed with other enzymes, with a peptide profile that varies by product. Casamino acids are acid-hydrolysed, almost entirely free amino acids with no tryptophan, for semi-defined media that need a known composition. When a formulation says tryptone, use tryptone rather than substituting the other two. For cell culture, choose a clear, low-endotoxin grade such as Tryptone N1.
Specifications
| Parameter | Specification |
|---|---|
| Appearance | Beige powder |
| Solubility in water at 2 % | Complete |
| pH (2 % solution) | 6.5 – 7.5 |
| Loss on drying | ≤ 8.0 % |
| Total nitrogen TN | 12.5 – 13.5% |
| Chlorides (NaCl) | ≤ 6 % |
| Total aerobic microbial count | ≤10 000 /g |
| Coliforms | ≤ 10 /g |
| Escherichia coli | Absence / g |
| Salmonella | Absence / 25 g |
| Staphylococcus aureus | Absence / 10 g |
| Yeasts and moulds | ≤ 100 /g |
Release specifications. Actual values are reported on the COA supplied with each batch.
Product highlights
- Food-grade raw materials, produced in an ISO 22000 certified facility
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Non-GMO
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Food grade versus laboratory grade
Most culture media suppliers use laboratory-grade raw materials, which are held to lower standards for heavy metal residues, microbial contamination control, and traceability. That is adequate for pure research but not for applications with stricter raw-material requirements. STBIO manufactures with food-grade raw materials throughout, in a facility certified to ISO 22000 food safety management.
- Food microbiology laboratories working to ISO 17025 or equivalent requirements
- Biotech manufacturers exporting fermentation products and needing full raw-material traceability
- Organisations with ESG or compliance audit requirements across their supply chain
- Companies sourcing both Halal and non-Halal products who need one consistent material grade
Frequently asked questions
01What is the difference between tryptone and peptone?
Peptone is the general term for protein hydrolysates; tryptone is one specific kind, made from casein with trypsin, so its peptide profile and tryptophan content are more defined. When a formulation specifies tryptone, do not substitute another peptone directly, as growth and yield may differ.
02Can I make LB medium from tryptone myself?
Yes. Standard LB Miller is 10 g tryptone, 5 g yeast extract and 10 g sodium chloride per litre; Lennox reduces sodium chloride to 5 g. Adjust to pH 7.0 and autoclave. To save preparation time or ensure batch consistency, use our ready-made LB Broth powder instead.
03Why is tryptone used for the indole test?
The indole test checks whether a bacterium can break tryptophan down to indole. Tryptone is high in tryptophan compared with other peptones, so tryptone water or tryptone broth made from it supplies enough substrate for a clear reaction, which is why it is the standard nitrogen source for this test.
04Does tryptone contain allergens? Can it be used in vegetarian or Halal formulations?
Tryptone is derived from milk casein, so it is a milk allergen and must be declared. It contains no meat, but as a dairy derivative it is not vegan; use soy or plant peptones for vegan formulations. For Halal requirements choose the certified version whose code ends in H.
05Which organisms is it suitable for?
Lactic acid bacteria. See the cultural response table and the specification sheet.
06Is the raw material non-GMO?
Yes. The raw material is non-GMO; the supplier declaration can be provided with the COA.
07Can it be used for food-grade applications?
Yes. It is made from food-grade raw materials in an ISO 22000 certified facility and is suitable for culturing starters for fermented products that are consumed directly.
08Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
09How should it be stored, and what is the shelf life?
When stored in sealed containers, at ambient temperature in dry and clean conditions, the expected shelf life is 2 years.
10How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Further reading
- What Are Casamino Acids? Casamino Acids vs Tryptone vs Casein PeptoneCasamino acids are casein hydrolysed completely with hydrochloric acid, consisting almost entirely of free amino acids. How they differ from tryptone and enzymatic casein peptone in hydrolysis, tryptophan, salt and use.
- Tryptose vs Tryptone: One Letter Apart, Different JobsTryptose is an enzymatic digest of mixed casein and meat protein used in media for fastidious organisms such as Brucella and Listeria. Tryptone is a pancreatic digest of casein alone, used in LB and TSB. Raw material, hydrolysis, specification and the formulations that call for each.
- What Is Peptone? Definition, Types, How It Is Made and How to ChoosePeptone is a mixture of peptides and amino acids made by hydrolysing protein, and the main nitrogen source in culture media. How it is made, the main types, tryptone versus peptone, and how to pick one for your organism.