Illustrative image only. Actual product may vary.Lactobacilli MRS Agar
Also known as：MRS Agar
The Lactobacilli MRS Agar is apt for the growth of lactic acid bacteria, including Lactobacillus, Streptococcus, Pediococcus and Leuconostoc.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 70.15 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Standard methods
- ISO 15214·IDF 149
Data updated：2026-11-07
Brand cross-reference：BD Difco Lactobacilli MRS Agar · Oxoid MRS Agar (de Man, Rogosa, Sharpe) · Merck MRS Agar（ISO 15214、IDF 149） Brand cross-reference
Overview
Lactobacilli MRS Agar is the solid form of the standard MRS formulation (de Man, Rogosa and Sharpe, 1960) for isolating, counting and picking single colonies of lactobacilli, streptococci, pediococci and leuconostocs. Polysorbate 80 supplies the fatty acids lactic acid bacteria need, sodium acetate and ammonium citrate suppress competitors, and manganese and magnesium ions promote growth. The basic plate for lactic acid bacteria counts in dairy, fermented foods and probiotic products.
Agar or broth: which to choose
MRS Agar is used for enumeration and single-colony isolation of lactic acid bacteria, the standard choice in fermented food and probiotic quality control. MRS Broth is used for strain activation, seed culture, and pre-fermentation scale-up. The two share the same formulation base and differ only in the solidifying agent.
Product highlights
- Manufactured to the standard formulation, 70.15 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How does food-grade MRS differ from standard MRS?
The food-grade version is manufactured from food-grade raw materials and replaces animal-derived nitrogen sources with plant- and yeast-based alternatives. It suits fermented foods and probiotic products that require Halal certification, vegetarian labelling, or full raw-material traceability. Performance is verified against standard control strains.
02How much powder per litre?
Use 70.15 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03What are the equivalent brand products and standard methods?
Cross-reference: BD Difco Lactobacilli MRS Agar, Oxoid MRS Agar (de Man, Rogosa, Sharpe), Merck MRS Agar. Standard methods: ISO 15214, IDF 149. Formulations may differ slightly between makers; refer to the specification sheet.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paperUsed in these applications
Used in published research and patents
- Particulate Cell Wall Materials of Lactobacillus acidophilus as Vaccine AdjuvantVeterinary Sciences 2022 · National Taipei University of TechnologyLactobacillus acidophilus cultured on MRS agar plates.
- Publications