Potato Dextrose Broth H
Also known as：PDB · PDB H · Potato Dextrose Broth (Halal) · Potato Dextrose Broth · Potato Glucose Broth
For the cultivation of yeasts and moulds, and for the preparation of fungal inocula.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 24 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
Overview
Potato Dextrose Broth H is the liquid form of PDA, for enriching, seed-culturing and fermenting yeasts and moulds, such as scaling up edible mycelium, yeasts and filamentous fungi, or liquid enrichment of yeasts and moulds from food and cosmetic samples. Potato-derived carbohydrates and glucose provide an ample carbon source. Halal-certified and made from food-grade raw materials.
Principle
Potato Dextrose Broth is the liquid form of the general-purpose medium widely used for the cultivation of yeasts and moulds.¹ Potato peptone provides nitrogen, peptides, carbohydrates, and the natural growth factors derived from potato, while dextrose serves as the carbon and energy source and supports luxuriant fungal growth. The naturally acidic reaction of the medium favours fungi over most bacteria; where bacterial contamination is a concern, the medium may be acidified with sterile tartaric acid or supplemented with antibiotics after sterilisation.²
Choosing a medium for yeasts and moulds
Potato Dextrose Agar and Sabouraud Dextrose Agar are the two general-purpose media most often used for yeasts and moulds. The former promotes mycelial growth and sporulation for morphological work; the latter's low pH itself suppresses bacteria. Malt-based media suit the cultivation and maintenance of yeasts, while the Czapek range is used for the taxonomy of filamentous fungi such as Aspergillus. Broths are for propagation, agars for enumeration and isolation.
Directions
Suspend 24 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Where greater selectivity is required, acidify the cooled medium to pH 3.5 with sterile 10% tartaric acid. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Candida albicans | 10231 | good-luxuriant |
| Saccharomyces cerevisiae | 9763 | good-luxuriant |
| Aspergillus brasiliensis | 16404 | good-luxuriant |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 24 g per litre
- Performance verified against standard control strains before release (Candida albicans, Saccharomyces cerevisiae, Aspergillus brasiliensis)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01Do I need to suppress bacterial growth in fungal testing?
It depends on the sample. For foods or environmental samples with high bacterial loads, the pH is usually lowered or antibiotics are added to suppress bacteria. Follow the requirements of your chosen method; we are happy to advise.
02How much powder per litre?
Use 24 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03Which organisms is it suitable for?
Yeast & Mold, Yeast, Mold, Candida, Aspergillus, Penicillium, S. cerevisiae, Candida albicans, Saccharomyces cerevisiae, Aspergillus brasiliensis. See the cultural response table and the specification sheet.
04How is this product prepared?
Suspend 24 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Where greater selectivity is required, acidify the cooled medium to pH 3.5 with sterile 10% tartaric acid. Test the finished medium for performance using stable, standard control cultures.
05How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
Used in published research and patents
- Method of preparing fermented food by using novel Rhizopus microsporus strain, and fermented foodEP Patent 4209134 B1 · TW Patent I805159 B · JP Patent 7637661 B2 2025 · Industrial Technology Research Institute (ITRI) · Granted in Europe, Taiwan and Japan; US application pendingPotato Dextrose Broth used to culture the isolated filamentous fungi and as the liquid base, with bean dregs, for fermentation by the Rhizopus microsporus strain.
- Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination TechniqueJournal of Visualized Experiments (JoVE) 2022 · National Sun Yat-sen UniversityPotato Dextrose Broth H listed in the materials table, used to culture the fungus for natural product analysis.
- Publications
References
- 1. United States Pharmacopeia, current edition. Potato Dextrose Agar, USP-NF, Rockville, MD.
- 2. U.S. Food and Drug Administration, Bacteriological Analytical Manual, Chapter 18, Yeasts, Moulds and Mycotoxins.