Illustrative image only. Actual product may vary.Burkholderia Cepacia Selective Agar Base
Also known as：BCSA Base · Burkholderia Cepacia Agar Base · BCSA · Burkholderia cepacia Selective Agar
For the selective isolation of Burkholderia cepacia complex from non-sterile pharmaceutical products and other materials, following the addition of a selective supplement.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 50.58 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-13
Overview
Principle
Burkholderia Cepacia Selective Agar was developed by Henry and co-workers as an improvement on earlier selective media, giving faster growth and better selectivity for the Burkholderia cepacia complex.¹ Pancreatic digest of casein and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and sodium chloride maintains the osmotic equilibrium. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Most strains of the Burkholderia cepacia complex oxidise these sugars slowly and release alkaline end-products from the peptones, so that colonies and the surrounding medium turn pink to red after prolonged incubation. Selectivity is provided by crystal violet, which inhibits Gram-positive organisms, vancomycin, which inhibits enterococci and other Gram-positive organisms not suppressed by crystal violet, and polymyxin B with gentamicin, which act together against other aerobic Gram-negative bacilli including Pseudomonas. The medium is specified in the pharmacopoeia for the examination of non-sterile products for the Burkholderia cepacia complex.²
Directions
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Burkholderia cepacia | 25416 | good-luxuriant |
| Pseudomonas aeruginosa | 9027 | inhibited |
| Escherichia coli | 8739 | inhibited |
| Staphylococcus aureus | 6538 | inhibited |
Product highlights
- Manufactured to the standard formulation, 50.58 g per litre
- Performance verified against standard control strains before release (Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 50.58 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Gram-negative, Pathogens, Aerobes, Burkholderia cepacia, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 50.58 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the reconstituted selective supplement. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. Henry D. A., Campbell M. E., LiPuma J. J. and Speert D. P., 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J. Clin. Microbiol., 35:614-619.
- 2. United States Pharmacopeia, current edition. Chapter <60> Microbiological Examination of Nonsterile Products — Tests for Burkholderia cepacia Complex, USP-NF, Rockville, MD.