Illustrative image only. Actual product may vary.ATCC Medium 271
Also known as：Escherichia Medium · ATCC 271 Medium · E. coli K-12 Medium
For the cultivation of Escherichia coli K-12 strains, as recommended by the American Type Culture Collection.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 20.3 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
Overview
Principle
The medium is the formulation recommended by the American Type Culture Collection for the cultivation of Escherichia coli K-12 and its derivatives.¹ Tryptone and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, dextrose provides a readily fermentable carbon and energy source, and sodium chloride maintains the osmotic equilibrium. Calcium chloride supplies calcium ions, and thiamine supports the growth of thiamine-requiring K-12 derivatives commonly used in laboratory work. The heat-sensitive components are supplied separately and added after sterilisation.
Directions
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Escherichia coli K-12 | 10798 | good-luxuriant |
Product highlights
- Manufactured to the standard formulation, 20.3 g per litre
- Performance verified against standard control strains before release (Escherichia coli K-12)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 20.3 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Gram-negative, Aerobes, E. coli, Escherichia coli K-12. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 19.0 g of Part A in 950 mL of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Dissolve 1.304 g of Part B in 50 mL of purified water and sterilize by filtration. Cool Part A and aseptically add the 50 mL of Part B. Mix well and dispense into sterile tubes or flasks. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. American Type Culture Collection, Catalogue of Bacteria and Bacteriophages, ATCC, Manassas, VA.