Illustrative image only. Actual product may vary.Brilliant-green Phenol-red Lactose Sucrose Agar
Also known as：BPLS · BPLS Agar · Brilliant Green Phenol Red Lactose Sucrose Agar · Brilliant Green Agar Modified · Modified Brilliant Green Agar
For the selective isolation of Salmonella species, other than Salmonella Typhi, from water, meat, milk, and other materials.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 51.69 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
Overview
Principle
The medium is a modification of the brilliant green agar of Kauffmann, with the addition of a phosphate buffer and a reduced concentration of brilliant green to improve the recovery of Salmonella.¹ Peptone, meat extract, and yeast extract supply nitrogen, amino acids, vitamins, and growth factors, and the phosphate buffer maintains the pH of the medium. Brilliant green inhibits Gram-positive bacteria and most Gram-negative bacilli other than Salmonella. Lactose and sucrose are the fermentable carbohydrates, and phenol red serves as the pH indicator. Salmonella does not ferment either sugar and forms red colonies surrounded by a bright red zone, whereas lactose- or sucrose-fermenting organisms produce acid and form yellow-green colonies with a yellow zone. The medium is specified in ISO 6340 for the detection of Salmonella in water and in DIN 10160 and DIN 10181.²
Directions
Suspend 51.69 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Salmonella enterica ser. Typhimurium | 14028 | good-luxuriant |
| Salmonella enterica ser. Enteritidis | 13076 | good-luxuriant |
| Escherichia coli | 25922 | inhibited-partial |
| Staphylococcus aureus | 25923 | inhibited |
Product highlights
- Manufactured to the standard formulation, 51.69 g per litre
- Performance verified against standard control strains before release (Salmonella enterica ser. Typhimurium, Salmonella enterica ser. Enteritidis, Escherichia coli, Staphylococcus aureus)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 51.69 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Enterobacteriaceae, Gram-negative, Pathogens, Salmonella, Salmonella enterica ser. Typhimurium, Salmonella enterica ser. Enteritidis, Escherichia coli, Staphylococcus aureus. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 51.69 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. Kauffmann F., 1935. Weitere Erfahrungen mit dem kombinierten Anreicherungsverfahren für Salmonellabacillen. Z. Hyg. Infektionskr., 117:26-32.
- 2. ISO 6340, Water quality — Detection of Salmonella spp., International Organization for Standardization, Geneva.