Illustrative image only. Actual product may vary.OGYE Agar Base
Also known as：OGYE · Oxytetracycline Glucose Yeast Extract Agar Base · OGY Agar Base · Mossel's OGYE Agar Base
For the selective enumeration of yeasts and moulds in food and other materials, following the addition of oxytetracycline.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 37 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
Overview
OGYE Agar Base (oxytetracycline glucose yeast extract agar) is a selective count plate for yeasts and moulds in food: yeast extract and glucose support fungal growth, oxytetracycline added before use suppresses bacteria, and the neutral pH lets injured yeasts recover better than on acidified media. Adopted by UK public health laboratories and many food methods as an alternative to acidified PDA. A base formulation; add oxytetracycline per the method.
Principle
OGYE Agar was described by Mossel and co-workers for the selective enumeration of yeasts and moulds in foods.¹ Yeast extract supplies B-group vitamins, nitrogen, and growth factors, and glucose provides the carbon and energy source. Unlike media that rely on acidification to suppress bacteria, OGYE Agar is used at a near-neutral pH, which avoids acid injury and improves the recovery of stressed yeasts and moulds from processed foods. Selectivity is provided instead by oxytetracycline, which inhibits a broad range of bacteria. For heavily contaminated samples, gentamicin may be added in addition to oxytetracycline to suppress resistant bacteria.²
Directions
Suspend 37 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C and aseptically add filter-sterilized oxytetracycline to give a final concentration of 100 mg/L. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Product highlights
- Manufactured to the standard formulation, 37 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 37 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Yeast, Mold, Candida, Aspergillus, Penicillium. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 37 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Avoid overheating. Cool to 45-50°C and aseptically add filter-sterilized oxytetracycline to give a final concentration of 100 mg/L. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. Mossel D. A. A., Kleynen-Semmeling A. M. C., Vincentie H. M., Beerens H. and Catsaras M., 1970. Oxytetracycline-glucose-yeast extract agar for selective enumeration of moulds and yeasts in foods and clinical material. J. Appl. Bacteriol., 33:454-457.
- 2. ISO 7954, Microbiology — General guidance for enumeration of yeasts and moulds — Colony count technique at 25°C, International Organization for Standardization, Geneva.