Illustrative image only. Actual product may vary.M-Broth
Also known as：M Broth · M Broth for Salmonella · Sperber and Deibel M Broth
For the post-enrichment of Salmonella species prior to detection by immunological methods.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 36.23 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
Overview
Principle
M-Broth was developed by Sperber and Deibel for the rapid detection of Salmonella in dried foods and feeds using broth cultures and serological reactions.¹ Yeast extract and pancreatic digest of casein supply nitrogen, amino acids, vitamins, and growth factors, and D-mannose serves as the carbon and energy source. Dipotassium hydrogen phosphate buffers the medium, and sodium chloride maintains the osmotic equilibrium. Manganese, magnesium, and iron supply essential ions, and polysorbate 80 provides fatty acids that stimulate growth. The medium supports rapid multiplication of Salmonella and the development of flagella, enhancing the expression of the flagellar antigens detected in immunoassays. It is used for a short post-enrichment step, typically 6 to 8 hours, between selective enrichment and immunological detection.²
Directions
Suspend 36.23 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Salmonella enterica ser. Typhimurium | 14028 | good-luxuriant |
| Salmonella enterica ser. Enteritidis | 13076 | good-luxuriant |
| Escherichia coli | 25922 | good-luxuriant |
Product highlights
- Manufactured to the standard formulation, 36.23 g per litre
- Performance verified against standard control strains before release (Salmonella enterica ser. Typhimurium, Salmonella enterica ser. Enteritidis, Escherichia coli)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 36.23 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Enterobacteriaceae, Gram-negative, Pathogens, Salmonella, Salmonella enterica ser. Typhimurium, Salmonella enterica ser. Enteritidis, Escherichia coli. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 36.23 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. Sperber W. H. and Deibel R. H., 1969. Accelerated procedure for Salmonella detection in dried foods and feeds involving only broth cultures and serological reactions. Appl. Microbiol., 17:533-539.
- 2. U.S. Food and Drug Administration, Bacteriological Analytical Manual, Chapter 5, Salmonella.