Illustrative image only. Actual product may vary.Motility Nitrate Medium, Buffered
Also known as：Buffered Motility Nitrate Medium · Motility Nitrate Buffered Medium · Nitrate Motility Medium
Motility Nitrate Medium, Buffered is used for the isolation and detection of Clostridium perfringens on the basis of motility and nitrate reduction.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 19.5 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
- Allergens
- Soy, Milk
Overview
Motility Nitrate Medium, Buffered is the semi-solid medium for confirming Clostridium perfringens: after stab inoculation, the non-motile C. perfringens grows only along the stab line, and a red colour on adding nitrate reagents shows reduction of nitrate to nitrite; the two results together separate it from other clostridia. Specified in the confirmation steps of the Taiwan FDA C. perfringens method, the FDA BAM and ISO 7937, and read alongside lactose-gelatin medium.
Principle
Peptone and enzymatic hydrolysate of animal tissue supply amino acids and other complex nitrogenous substances. Agar is added to obtain a semisolid gel that demonstrates motility of the organism along the stab line of inoculation; growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow only in the inoculated area. The medium contains glycerol and galactose to improve the consistency of the nitrate reduction reaction across different strains. In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present, indicating that nitrate reduction has occurred. Some organisms further reduce nitrite to ammonia, which can be detected by adding a small amount of zinc dust to tubes showing no color.
What biochemical identification media are for
Biochemical identification media reveal whether a strain can use a particular substrate, read through colour change, gas production, or a zone of clearing. They remain the basis of conventional identification and confirmatory quality control. Each medium covers one characteristic, so several are normally read together alongside known positive and negative control strains.
Directions
Suspend 19.5 g of powder in 1 L of purified water containing 5 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into test tubes to make them half full. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Product highlights
- Manufactured to the standard formulation, 19.5 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 16: Clostridium perfringensBAM Chapter 16Confirmation / biochemicalNamed in the method as：Motility-nitrate medium, buffered
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 16: Clostridium perfringens（BAM Chapter 16）: Confirmation / biochemical. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02How much powder per litre?
Use 19.5 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03How is this product prepared?
Suspend 19.5 g of powder in 1 L of purified water containing 5 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into test tubes to make them half full. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Further reading
- FDA BAM Culture Media Cross-Reference: Which Media Each BAM Chapter Uses and the STBIO EquivalentAerobic plate count, coliforms and E. coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Vibrio, Clostridium perfringens, yeasts and moulds and Cronobacter: the media named in each FDA Bacteriological Analytical Manual chapter, matched to Taiwan-made dehydrated media for US laboratories qualifying a second source.
- Which Culture Media Do Taiwan FDA Food Microbiology Methods Specify? Cross-Reference for 21 Announced MethodsAerobic plate count, coliforms, E. coli, Enterobacteriaceae, Salmonella, Staphylococcus aureus, Listeria monocytogenes, lactic acid bacteria, yeasts and moulds, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens and Cronobacter: the media named in each Taiwan FDA (TFDA) announced method, matched to Taiwan-made products.