Standard Nutrient Agar No. 1
Also known as：SNA · Standard I Nutrient Agar · Std I Agar · Standard 1 NA · Nutrient Agar No. 1
Standard Nutrient Agar No. 1 is used as a general-purpose medium for the cultivation, maintenance, and enumeration of non-fastidious microorganisms.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 37 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
- Standard methods
- ISO 11133
Data updated：2026-11-07
Brand cross-reference：Oxoid Standard Plate Count Agar · Merck Standard I Nutrient Agar（ISO 11133） Brand cross-reference
- Allergens
- Milk, Soy
Overview
Standard Nutrient Agar No. 1 is an agar (solid) medium, a standard general-purpose and enumeration formulation. It is a general-purpose count medium to the European standard formulation for non-fastidious bacteria. STBIO manufactures it from food-grade raw materials in an ISO 22000 certified facility, with a COA for every batch. This is the Halal-certified version.
Principle
Peptone, yeast extract, and meat extract provide nitrogen, vitamins, amino acids, and carbon required for the growth of non-fastidious organisms. Sodium chloride maintains the osmotic equilibrium of the medium. The addition of 10% biological fluids such as blood, serum, or egg yolk makes the medium suitable for the cultivation of related fastidious organisms. The medium may also be used as a base for the preparation of special-purpose culture media.
Choosing a general-purpose or enumeration medium
Plate Count Agar is used for total viable counts in food, water, and environmental samples. Nutrient Broth and Tryptic Soy media are the general-purpose choices for non-fastidious bacteria, with the latter richer and also used for sterility testing and environmental monitoring. Brain Heart Infusion is the richest of the three and suits fastidious organisms such as streptococci. Use the agar form for enumeration and the broth for propagation.
Directions
Suspend 37 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. Test the finished medium for performance using stable, standard control cultures.
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 37 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01Which medium should I use for total viable counts?
Total viable counts in food and water normally use Plate Count Agar with the pour-plate method at 30 °C. Follow the incubation conditions specified by the method you are working to.
02How much powder per litre?
Use 37 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03What are the equivalent brand products and standard methods?
Cross-reference: Oxoid Standard Plate Count Agar, Merck Standard I Nutrient Agar. Standard methods: ISO 11133. Formulations may differ slightly between makers; refer to the specification sheet.
04How is this product prepared?
Suspend 37 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes. Test the finished medium for performance using stable, standard control cultures.
05How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paper