Lactose Broth
Also known as：LB (Lactose) · Fluid Lactose Medium
For the detection of coliform organisms in water and dairy products, and for the pre-enrichment of Salmonella from foods.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 13 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- ISO 22000 · Halal
Data updated：2026-11-06
- Allergens
- Milk
Overview
Lactose Broth is the traditional presumptive broth for coliforms and the non-selective pre-enrichment broth for Salmonella in earlier FDA BAM methods. Peptone and beef extract supply nitrogen, and lactose lets coliforms produce acid and gas. Used for coliform MPN presumptive tests on food, dairy and water, Salmonella pre-enrichment, and characterising lactose fermentation.
Principle
Lactose Broth is a non-selective medium used in the multiple-tube fermentation technique for the presumptive detection of coliform organisms.¹ Beef extract and peptone supply nitrogen, amino acids, vitamins, and growth factors, and lactose serves as the fermentable carbohydrate. Coliforms ferment lactose with the production of acid and gas; the accumulation of gas in the Durham tube within 48 hours constitutes a positive presumptive test, which must be confirmed on a selective medium. Because the medium contains no inhibitory agents, it also allows the resuscitation of sub-lethally injured cells, and is specified as a pre-enrichment medium for the recovery of Salmonella from foods before selective enrichment.²
Media for anaerobes and fastidious organisms
Anaerobes and fastidious organisms require media containing reducing agents, blood, or infusion components, together with an anaerobic incubation environment. Some formulations need heat-labile supplements such as blood, serum, or antibiotics added after sterilisation; follow the preparation instructions for the timing and method.
Directions
Suspend 13 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes, with inverted Durham tubes where gas production is to be observed. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Klebsiella aerogenes | 25922: 13048 · good-luxuriant: good-luxuriant · positive: positive | |
| Salmonella enterica ser. Typhimurium | 25922: 14028 · good-luxuriant: good-luxuriant · positive: negative |
Product highlights
- Food-grade raw materials, produced in an ISO 22000 certified facility
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 13 g per litre
- Performance verified against standard control strains before release (Klebsiella aerogenes, Salmonella enterica ser. Typhimurium)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Food grade versus laboratory grade
Most culture media suppliers use laboratory-grade raw materials, which are held to lower standards for heavy metal residues, microbial contamination control, and traceability. That is adequate for pure research but not for applications with stricter raw-material requirements. STBIO manufactures with food-grade raw materials throughout, in a facility certified to ISO 22000 food safety management.
- Food microbiology laboratories working to ISO 17025 or equivalent requirements
- Biotech manufacturers exporting fermentation products and needing full raw-material traceability
- Organisations with ESG or compliance audit requirements across their supply chain
- Companies sourcing both Halal and non-Halal products who need one consistent material grade
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 5: SalmonellaBAM Chapter 5Pre-enrichmentNamed in the method as：Lactose broth
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 5: Salmonella（BAM Chapter 5）: Pre-enrichment. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02How much powder per litre?
Use 13 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03Which organisms is it suitable for?
Coliforms, Gram-negative, E. coli, Salmonella, Klebsiella aerogenes, Salmonella enterica ser. Typhimurium. See the cultural response table and the specification sheet.
04Can it be used for food-grade applications?
Yes. It is made from food-grade raw materials in an ISO 22000 certified facility and is suitable for culturing starters for fermented products that are consumed directly.
05How is this product prepared?
Suspend 13 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes, with inverted Durham tubes where gas production is to be observed. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
06How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
Further reading
- FDA BAM Culture Media Cross-Reference: Which Media Each BAM Chapter Uses and the STBIO EquivalentAerobic plate count, coliforms and E. coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Vibrio, Clostridium perfringens, yeasts and moulds and Cronobacter: the media named in each FDA Bacteriological Analytical Manual chapter, matched to Taiwan-made dehydrated media for US laboratories qualifying a second source.
- Which Culture Media Do Taiwan FDA Food Microbiology Methods Specify? Cross-Reference for 21 Announced MethodsAerobic plate count, coliforms, E. coli, Enterobacteriaceae, Salmonella, Staphylococcus aureus, Listeria monocytogenes, lactic acid bacteria, yeasts and moulds, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens and Cronobacter: the media named in each Taiwan FDA (TFDA) announced method, matched to Taiwan-made products.
References
- 1. American Public Health Association, Standard Methods for the Examination of Water and Wastewater, current edition, APHA Press, Washington, D.C.
- 2. U.S. Food and Drug Administration, Bacteriological Analytical Manual, Chapter 5, Salmonella.