Brilliant Green Bile Broth 2%
Also known as：BGLB · BGBLB · BGBB · Brilliant Green Bile Lactose Broth
Brilliant Green Bile Broth 2% is used for the detection or confirmation of coliform bacteria in water, food, and or dairy products in a laboratory setting, not intended for use in the diagnosis of disease or other conditions in humans.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 40.01 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
- Standard methods
- ISO 4831·APHA Standard Methods
Data updated：2026-11-06
Brand cross-reference：BD Difco Brilliant Green Bile 2% · Oxoid Brilliant Green Bile (2%) Broth · Merck Brilliant-green Bile Lactose Broth（ISO 4831、APHA Standard Methods） Brand cross-reference
Overview
Brilliant Green Bile Broth 2% (BGLB) is the confirmation broth for the coliform MPN method: 2% ox bile and brilliant green suppress Gram-positive and spore-forming bacteria, and lactose fermentation is read as gas in the Durham tube. The Taiwan FDA coliform method transfers LST presumptive-positive tubes to BGLB, and only tubes producing gas after 48 ± 2 h at 35 °C count as coliform-positive; ISO 4831 and APHA Standard Methods for water also specify it. Food-grade raw materials, Halal certified.
How selective media fit into the testing workflow
Pathogen testing normally runs in three stages: a pre-enrichment medium such as Buffered Peptone Water repairs injured cells and raises numbers, a selective enrichment then suppresses competing flora, and finally a selective differential agar isolates the target colonies. Selective agents are typically bile salts, dyes, or antibiotics; differential agents make target colonies develop a characteristic colour or morphology. Choose the combination specified by the method you follow.
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 40.01 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
FDA BAM methods
This product corresponds to a medium used in the chapters of the US FDA Bacteriological Analytical Manual (BAM), the reference methods for FDA regulatory testing. Follow the current online edition of each chapter.
- FDA BAM Chapter 4: Enumeration of Escherichia coli and the Coliform BacteriaBAM Chapter 4Confirmation / biochemicalNamed in the method as：Brilliant green lactose bile (BGLB) broth, 2%
BAM specifies methods and media formulations; it does not certify brands. Add supplements as the chapter requires, and verify media against reference strains under your quality system.
Frequently asked questions
01Does this medium meet FDA BAM methods?
BAM specifies methods and formulations, not brands. This product corresponds to a medium used in: FDA BAM Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria（BAM Chapter 4）: Confirmation / biochemical. It is manufactured to the standard formulation and ships with a COA per batch; add supplements as the chapter requires and follow the current online edition.
02Can selective media be autoclaved?
Not always. Some media containing dyes or selective inhibitors lose their selectivity if overheated and should only be heated until fully dissolved, without autoclaving. Follow the preparation instructions for this product, and contact us if you are unsure.
03How much powder per litre?
Use 40.01 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
04Which organisms is it suitable for?
Coliforms, Gram-negative, E. coli, Gram-positive. See the cultural response table and the specification sheet.
05What are the equivalent brand products and standard methods?
Cross-reference: BD Difco Brilliant Green Bile 2%, Oxoid Brilliant Green Bile (2%) Broth, Merck Brilliant-green Bile Lactose Broth. Standard methods: ISO 4831, APHA Standard Methods. Formulations may differ slightly between makers; refer to the specification sheet.
06How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
Further reading
- FDA BAM Culture Media Cross-Reference: Which Media Each BAM Chapter Uses and the STBIO EquivalentAerobic plate count, coliforms and E. coli, Salmonella, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Vibrio, Clostridium perfringens, yeasts and moulds and Cronobacter: the media named in each FDA Bacteriological Analytical Manual chapter, matched to Taiwan-made dehydrated media for US laboratories qualifying a second source.
- Which Culture Media Do Taiwan FDA Food Microbiology Methods Specify? Cross-Reference for 21 Announced MethodsAerobic plate count, coliforms, E. coli, Enterobacteriaceae, Salmonella, Staphylococcus aureus, Listeria monocytogenes, lactic acid bacteria, yeasts and moulds, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens and Cronobacter: the media named in each Taiwan FDA (TFDA) announced method, matched to Taiwan-made products.