Bifidobacterium Agar
Bifidobacterium Agar is used for the cultivation, isolation, and maintenance of various Bifidobacterium species.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 49.3 g
- pH (25 °C)
- 6.8 ± 0.5
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
- Storage
- Store at 2–8 °C in sealed, dry, clean conditions.
- Shelf life
- 36 months from manufacture date when stored as directed.
Overview
Bifidobacterium Agar is an agar (solid) medium. It is used for selective isolation and counting of bifidobacteria under strict anaerobiosis. STBIO manufactures it from food-grade raw materials in an ISO 22000 certified facility, with a COA for every batch. This is the Halal-certified version.
Principle
Bifidobacterium species are strictly anaerobic, Gram-positive rods that form one of the predominant bacterial populations of the human intestinal tract and are widely used as probiotic cultures in fermented dairy products.¹ Bifidobacterium Agar supports their cultivation and maintenance. Special peptone supplies nitrogen, amino acids, peptides, and the growth factors required by these nutritionally demanding organisms. Dextrose provides the carbon and energy source, and sodium chloride maintains the osmotic equilibrium of the medium. Soluble starch acts as a protective colloid, adsorbing toxic metabolites and shielding the cells from inhibitory substances in the medium. L-Cysteine hydrochloride functions as a reducing agent, lowering the redox potential and establishing the reduced conditions required for the growth of bifidobacteria.² Because the organisms are strict anaerobes, plates must be incubated under anaerobic conditions.
Specifications
| Parameter | Specification |
|---|---|
| pH (at 25°C) | 6.8 ± 0.5 |
Release specifications. Actual values are reported on the COA supplied with each batch.
Directions
Suspend 49.3 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Incubate under anaerobic conditions. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Bifidobacterium bifidum | 15696 | Good |
| Bifidobacterium breve | 15698 | Good |
| Bifidobacterium infantis | 25962 | Good |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 49.3 g per litre, pH 6.8 ± 0.5
- Performance verified against standard control strains before release (Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 49.3 g per litre of deionised water; pH after sterilisation 6.8 ± 0.5. Preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Anaerobes, Gram-positive, Probiotics, Bifidobacterium, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 49.3 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. Incubate under anaerobic conditions. Test the finished medium for performance using stable, standard control cultures.
04Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
05How should it be stored, and what is the shelf life?
Store at 2–8 °C in sealed, dry, clean conditions. 36 months from manufacture date when stored as directed.
06How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
References
- 1. Scardovi V., 1986. Genus Bifidobacterium, in Bergey's Manual of Systematic Bacteriology, Vol. 2, Williams & Wilkins, Baltimore.
- 2. Atlas R. M., 2010. Handbook of Microbiological Media, 4th Ed., CRC Press, Boca Raton.