Mannitol Salt Broth
Also known as：MSB · Chapman Broth · Mannitol Salt Medium
Mannitol Salt Broth is a selective medium used for the isolation of Staphylococci and the detection of Staphylococcus aureus from clinical specimens.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 96.03 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
- Allergens
- Milk
Overview
Mannitol Salt Broth is the liquid form of MSA: its 7.5% salt lets only salt-tolerant staphylococci grow, and Staphylococcus aureus ferments mannitol, turning phenol red yellow. Used for selective enrichment of S. aureus from food, cosmetic and clinical samples before plating for isolation and confirmation.
How selective media fit into the testing workflow
Pathogen testing normally runs in three stages: a pre-enrichment medium such as Buffered Peptone Water repairs injured cells and raises numbers, a selective enrichment then suppresses competing flora, and finally a selective differential agar isolates the target colonies. Selective agents are typically bile salts, dyes, or antibiotics; differential agents make target colonies develop a characteristic colour or morphology. Choose the combination specified by the method you follow.
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 96.03 g per litre
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01Can selective media be autoclaved?
Not always. Some media containing dyes or selective inhibitors lose their selectivity if overheated and should only be heated until fully dissolved, without autoclaving. Follow the preparation instructions for this product, and contact us if you are unsure.
02How much powder per litre?
Use 96.03 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
03How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.