Mueller Hinton Agar
Also known as：MHA · M-H AGAR
Mueller Hinton Agar is used for antimicrobial susceptibility testing (AST) of aerobic and facultative anaerobic bacteria in accordance with CLSI and EUCAST standards.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 38 g
- pH (25 °C)
- 7.3 ± 0.5
- Appearance
- Beige powder
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
- Standard methods
- CLSI M02·EUCAST disk diffusion
Data updated：2026-11-07
Brand cross-reference：BD Difco Mueller Hinton Agar · Oxoid Mueller-Hinton Agar · Merck Mueller-Hinton Agar（CLSI M02、EUCAST disk diffusion） Brand cross-reference
- Storage
- Store at 10–30 °C in sealed, dry, clean conditions.
- Shelf life
- 36 months from manufacture date when stored as directed.
Overview
Mueller Hinton Agar is the international standard medium for antimicrobial susceptibility testing by disc diffusion, as specified by both CLSI and EUCAST. The formulation deliberately avoids components that interfere with antibiotics, starch adsorbs toxic metabolites, and the agar content gives uniform antibiotic diffusion so inhibition zones can be read against standard tables. Used for aerobic and facultative anaerobic bacteria, with performance verified against CLSI QC strains before release.
Principle
Acid hydrolysate of casein and beef extract supply amino acids and other nitrogenous substances, minerals and other nutrients to support the growth of microorganisms. Starch acts as a protective colloid against toxic substances that may be present in the medium. Hydrolysis of starch during autoclaving provides a small amount of dextrose, which is a source of energy. Agar is the solidifying agent.
Media for anaerobes and fastidious organisms
Anaerobes and fastidious organisms require media containing reducing agents, blood, or infusion components, together with an anaerobic incubation environment. Some formulations need heat-labile supplements such as blood, serum, or antibiotics added after sterilisation; follow the preparation instructions for the timing and method.
Specifications
| Parameter | Specification |
|---|---|
| Appearance | Beige powder |
| pH (at 25°C) | 7.3 ± 0.5 |
Release specifications. Actual values are reported on the COA supplied with each batch.
Directions
Suspend 38 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121 °C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Enterococcus faecalis | 33186 | |
| Escherichia coli | 25922 | |
| Escherichia coli | 35218 | |
| Pseudomonas aeruginosa | 27853 | |
| Staphylococcus aureus | 25923 | |
| Staphylococcus aureus | 43300 |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 38 g per litre, pH 7.3 ± 0.5
- Performance verified against standard control strains before release (Enterococcus faecalis, Escherichia coli, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus aureus)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 38 g per litre of deionised water; pH after sterilisation 7.3 ± 0.5. Preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. See the cultural response table and the specification sheet.
03What are the equivalent brand products and standard methods?
Cross-reference: BD Difco Mueller Hinton Agar, Oxoid Mueller-Hinton Agar, Merck Mueller-Hinton Agar. Standard methods: CLSI M02, EUCAST disk diffusion. Formulations may differ slightly between makers; refer to the specification sheet.
04How is this product prepared?
Suspend 38 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121 °C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
05Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
06How should it be stored, and what is the shelf life?
Store at 10–30 °C in sealed, dry, clean conditions. 36 months from manufacture date when stored as directed.
07How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.