Illustrative image only. Actual product may vary.Pseudomonas Agar Base
Also known as：Pseudomonas CFC Agar Base · Pseudomonas CN Agar Base · Pseudomonas Selective Agar Base · CFC Agar Base
For the isolation and enumeration of Pseudomonas aeruginosa in water and of Pseudomonas species in meat, milk, and dairy products, following the addition of glycerol and a selective supplement.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 48.4 g
- Pack sizes
- 500 g · 5 kg · 25 kg
Data updated：2026-11-06
Overview
Principle
The base is derived from King's Medium A and contains gelatin peptone and casein hydrolysate as sources of nitrogen, amino acids, and growth factors, with glycerol as the carbon and energy source. Potassium sulphate and magnesium chloride enhance the production of pigments by Pseudomonas aeruginosa. With the CFC supplement, cetrimide, fusidic acid, and cefalotin suppress the competing flora of meat and dairy products, allowing the selective enumeration of Pseudomonas species, which are important spoilage organisms of chilled foods.¹ With the CN supplement, the higher concentration of cetrimide together with nalidixic acid makes the medium selective for Pseudomonas aeruginosa, which is enumerated in water by membrane filtration.² Colonies producing the blue-green pigment pyocyanin are regarded as confirmed Pseudomonas aeruginosa, while fluorescent colonies without pyocyanin require further confirmation. The medium is specified in ISO 16266 for water, ISO 13720 for meat, and ISO/TS 11059 for milk and milk products.³
Directions
Suspend 48.4 g of powder in 1 L of purified water containing 10 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the CFC selective supplement for Pseudomonas species, or the CN selective supplement for Pseudomonas aeruginosa. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Pseudomonas fluorescens | 13525 | good-luxuriant |
| Pseudomonas aeruginosa | 27853 | good-luxuriant |
| Pseudomonas aeruginosa | 27853 | good-luxuriant |
| Escherichia coli | 25922 | inhibited |
Product highlights
- Manufactured to the standard formulation, 48.4 g per litre
- Performance verified against standard control strains before release (Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Escherichia coli)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 48.4 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Gram-negative, Psychrotrophs, Pathogens, Pseudomonas, Pseudomonas fluorescens, Pseudomonas aeruginosa, Escherichia coli. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 48.4 g of powder in 1 L of purified water containing 10 mL of glycerol. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the CFC selective supplement for *Pseudomonas* species, or the CN selective supplement for *Pseudomonas aeruginosa*. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
References
- 1. Mead G. C. and Adams B. W., 1977. A selective medium for the rapid isolation of pseudomonads associated with poultry meat spoilage. Br. Poult. Sci., 18:661-670.
- 2. ISO 16266, Water quality — Detection and enumeration of Pseudomonas aeruginosa — Method by membrane filtration, International Organization for Standardization, Geneva.
- 3. ISO 13720, Meat and meat products — Enumeration of presumptive Pseudomonas spp., International Organization for Standardization, Geneva.