LB Broth Miller H
Also known as：LB · LB Broth · Miller (Halal) · LB Broth Miller · Luria-Bertani Broth Miller · Lysogeny Broth Miller · LB Miller
For the cultivation and maintenance of Escherichia coli and other recombinant strains in molecular biology applications.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 25 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
Brand cross-reference：BD Difco LB Broth, Miller · Oxoid LB Broth (Miller) · Merck LB Broth (Miller) Brand cross-reference
Overview
LB Broth Miller H is the Halal certified version of LB Broth Miller, identical in formulation and intended use, manufactured from food-grade raw materials with Halal documentation. It supports the cultivation and maintenance of Escherichia coli and recombinant strains for biomanufacturing, food-grade fermentation, and contract production where Halal certification is required.
Principle
LB Broth, Miller is a nutrient-rich medium widely used for the cultivation of Escherichia coli in molecular biology and recombinant DNA work.¹ Tryptone provides nitrogen, amino acids, and peptides, while yeast extract supplies B-complex vitamins, nucleotides, and additional nitrogen and carbon sources. Sodium chloride maintains the osmotic balance and provides sodium ions for membrane transport. The Miller formulation contains 10 g/L of sodium chloride, a higher concentration than the Lennox and Luria formulations, making it suitable for applications requiring higher ionic strength.² The medium supports rapid, high-density growth and is routinely used for the propagation of plasmid-bearing strains and the preparation of competent cells.
Choosing between Miller, Lennox, and low-salt formulations
The LB formulations differ mainly in sodium chloride content. Miller carries the highest level and is the default for most standard E. coli workflows; Lennox carries less and suits electroporation or osmotically sensitive strains. STBIO offers several further LB variants — contact us if you need a specific salt level or raw-material specification.
Directions
Suspend 25 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Escherichia coli | 25922 | good-luxuriant |
| Escherichia coli | 8739 | good-luxuriant |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 25 g per litre
- Performance verified against standard control strains before release (Escherichia coli, Escherichia coli)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01What is the difference between LB Broth Miller and Lennox?
The main difference is the sodium chloride level: Miller contains more than Lennox. Miller is the default for most standard E. coli workflows, while Lennox suits electroporation and osmotically sensitive strains.
02Which LB medium should I use for transformation?
For chemical (heat-shock) transformation, the recovery step normally uses LB Broth Miller or Lennox, incubating at 37 °C for 45–60 minutes before plating. For electroporation, the low-salt Lennox formulation is recommended to avoid arcing.
03How does LB Broth differ from Nutrient Broth?
Both are general-purpose liquid media but differ in composition. LB Broth combines tryptone, yeast extract, and sodium chloride and is optimised for E. coli and recombinant strains, making it the standard in molecular biology laboratories. Nutrient Broth is simpler and suits routine cultivation but is not tailored to recombinant work.
04How much powder per litre?
Use 25 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
05Which organisms is it suitable for?
Gram-negative, Aerobes, E. coli, Escherichia coli. See the cultural response table and the specification sheet.
06What are the equivalent brand products and standard methods?
Cross-reference: BD Difco LB Broth, Miller, Oxoid LB Broth (Miller), Merck LB Broth (Miller). Standard methods: . Formulations may differ slightly between makers; refer to the specification sheet.
07How is this product prepared?
Suspend 25 g of powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Dispense into tubes or flasks. Sterilize by autoclaving at 121°C for 15 minutes. Test the finished medium for performance using stable, standard control cultures.
08Is there a non-Halal version?
Yes. This is the Halal certified version; an equivalent standard version is also available.
09How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paperUsed in these applications
Further reading
References
- 1. Bertani G., 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol., 62:293-300.
- 2. Miller J. H., 1972. Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.