LB Medium, Modified (Buffered)
Also known as：LB
LB Medium, Modified (Buffered) is used for cultivation of Escherichia coli and related bacteria, recombinant protein expression studies, plasmid propagation and molecular cloning, seed culture preparation for fermentation, and general microbiological research.
- Category
- Dehydrated Culture Media
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
- Allergens
- Soy
Choosing between Miller, Lennox, and low-salt formulations
The LB formulations differ mainly in sodium chloride content. Miller carries the highest level and is the default for most standard E. coli workflows; Lennox carries less and suits electroporation or osmotically sensitive strains. STBIO offers several further LB variants — contact us if you need a specific salt level or raw-material specification.
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01What is the difference between LB Broth Miller and Lennox?
The main difference is the sodium chloride level: Miller contains more than Lennox. Miller is the default for most standard E. coli workflows, while Lennox suits electroporation and osmotically sensitive strains.
02Which LB medium should I use for transformation?
For chemical (heat-shock) transformation, the recovery step normally uses LB Broth Miller or Lennox, incubating at 37 °C for 45–60 minutes before plating. For electroporation, the low-salt Lennox formulation is recommended to avoid arcing.
03How does LB Broth differ from Nutrient Broth?
Both are general-purpose liquid media but differ in composition. LB Broth combines tryptone, yeast extract, and sodium chloride and is optimised for E. coli and recombinant strains, making it the standard in molecular biology laboratories. Nutrient Broth is simpler and suits routine cultivation but is not tailored to recombinant work.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Academic citation
Products from this range have been cited as the culture media source in a peer-reviewed study by a National Taiwan University research team:
Chan et al. (2024). Single-strain probiotics enhance growth, anti-pathogen immunity, and resistance to Nocardia seriolae in grey mullet (Mugil cephalus) via gut microbiota modulation. Animal Microbiome, 6, 67. Springer Nature.
Media attribution: Tryptone Soy Broth, MRS medium, LB broth, beef extract, peptone, yeast extract, agar (STBIO, New Taipei, Taiwan)
Read the full paper