Marine Agar 2216
Also known as：MA 2216 · Zobell Marine Agar · Zobell Marine Agar 2216 · Marine Agar · ZMA
For the cultivation, isolation, and enumeration of heterotrophic marine bacteria.
- Category
- Dehydrated Culture Media
- Dissolve per litre
- 55.25 g
- Pack sizes
- 500 g · 5 kg · 25 kg
- Certifications
- Halal
Data updated：2026-11-06
Overview
Marine Agar 2216 is an agar (solid) medium. It is with a salt profile close to seawater, used to isolate and cultivate marine bacteria. STBIO manufactures it from food-grade raw materials in an ISO 22000 certified facility, with a COA for every batch. This is the Halal-certified version.
Principle
Marine Agar 2216 is formulated according to the composition described by ZoBell to reproduce the major mineral content of seawater, thereby supporting the abundant growth of marine bacteria.¹ The medium contains a high concentration of sodium chloride together with magnesium, calcium, potassium, and trace minerals that approximate the ionic composition of natural seawater.² Peptone and yeast extract serve as the sources of nitrogen, carbon, amino acids, and vitamins, while ferric citrate supplies iron required by many marine organisms. Because most marine bacteria are heterotrophic and sensitive to elevated temperatures, the medium is cooled to 42°C before pour-plate inoculation to preserve viability. Both pour plate and spread plate techniques may be used for enumeration.
Directions
Suspend 55.3 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. For the pour plate technique, cool the medium to 42°C before inoculation, as most marine bacteria are thermosensitive. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
Cultural response
| Test organism | ATCC | Growth |
|---|---|---|
| Vibrio fischeri | 7744 | Good |
| Vibrio harveyi | 14126 | Good |
Product highlights
- Halal certified, suitable for the Malaysian, Indonesian, and Middle Eastern markets
- Manufactured to the standard formulation, 55.25 g per litre
- Performance verified against standard control strains before release (Vibrio fischeri, Vibrio harveyi)
- Available in 500 g / 5 kg / 25 kg, from laboratory to industrial scale
- COA, specification sheet, and technical data sheet available; samples on request
- Manufactured in Taiwan with reliable supply
Frequently asked questions
01How much powder per litre?
Use 55.25 g per litre of deionised water; preparation and sterilisation conditions are given on the specification sheet.
02Which organisms is it suitable for?
Vibrio fischeri, Vibrio harveyi. See the cultural response table and the specification sheet.
03How is this product prepared?
Suspend 55.3 g of the powder in 1 L of purified water. Heat with continuous stirring and bring to the boil for 1 minute. Sterilize by autoclaving at 121°C for 15 minutes. For the pour plate technique, cool the medium to 42°C before inoculation, as most marine bacteria are thermosensitive. Mix well and pour into sterile Petri plates. Test the finished medium for performance using stable, standard control cultures.
04How do I request a bulk quotation, and can you provide a COA?
Yes. This product is available in 500 g, 5 kg, 25 kg. Tell us your application and volume when requesting a quotation; we reply within one working day and can supply the COA, specification sheet, and technical data sheet.
Used in these applications
References
- 1. ZoBell C. E., 1941. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res., 4:42-75.
- 2. Lyman J. and Fleming R. H., 1940. Composition of sea water. J. Mar. Res., 3:134-146.